| Downregulation of survivin expression and elevation of caspase-3 activity involved in pitavastatin-induced HepG 2 cell apoptosis. | |
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MedLine Citation:
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PMID: 17611660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to research the apoptosis of human hepatocellular carcinoma cell line HepG 2 induced by pitavastatin. HepG 2 cells were treated with increasing doses of pitavastatin or with mevalonic acid for 48 h. The proliferation of cells was detected with WST-8. The morphology of the nucleus was observed under a microscope by Hoechst 33258 staining. The apoptosis peaks were examined by flow cytometry. The expression of survivin mRNA was examined with RT-PCR. The caspase-3 activity was detected with caspase-3 colorimetric protease assay. We found that growth inhibitory effects were observed for treatment with pitavastatin at 10-50 microM. Pitavastatin at 10 microM induced granular apoptotic bodies of HepG 2 cells. Furthermore, pitavastatin at 10 microM increased the appearance of sub-G1 population of HepG 2 cells. Finally, pitavastatin at 10 microM downregulated the expression of survivin mRNA and upregulated the caspase-3 activity, which was clearly related to the HMG-CoA reductase activity. These results suggest that pitavastatin at 10 microM induces apoptosis of HepG 2 cells, which is associated with the decreased expression of survivin mRNA and increased caspase-3 activity of HepG 2 cells. |
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Authors:
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Juyong Wang; Zhenye Xu; Ming Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology reports Volume: 18 ISSN: 1021-335X ISO Abbreviation: Oncol. Rep. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-05 Completed Date: 2007-09-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 383-7 Citation Subset: IM |
Affiliation:
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Tumor Research Institute, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, PR China. wangjuyong1@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Bisbenzimidazole / chemistry Caspase 3 / metabolism Cell Line, Tumor Cell Nucleus / chemistry, drug effects, metabolism Down-Regulation / drug effects, genetics Enzyme Activation / drug effects Flow Cytometry G1 Phase / drug effects Gene Expression Regulation, Neoplastic / drug effects* Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology Mevalonic Acid / pharmacology Microscopy, Fluorescence Microtubule-Associated Proteins / genetics* Neoplasm Proteins / genetics* Quinolines / pharmacology* RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/BIRC5 protein, human; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/Quinolines; 0/RNA, Messenger; 147526-32-7/NK 104; 150-97-0/Mevalonic Acid; 23491-44-3/Bisbenzimidazole; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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