| Downregulation of miR-122 in the rodent and human hepatocellular carcinomas. | |
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MedLine Citation:
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PMID: 16924677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MicroRNAs (miRs) are conserved small non-coding RNAs that negatively regulate gene expression. The miR profiles are markedly altered in cancers and some of them have a causal role in tumorigenesis. Here, we report changes in miR expression profile in hepatocellular carcinomas (HCCs) developed in male Fisher rats-fed folic acid, methionine, and choline-deficient (FMD) diet. Comparison of the miR profile by microarray analysis showed altered expression of some miRs in hepatomas compared to the livers from age-matched rats on the normal diet. While let-7a, miR-21, miR-23, miR-130, miR-190, and miR-17-92 family of genes was upregulated, miR-122, an abundant liver-specific miR, was downregulated in the tumors. The decrease in hepatic miR-122 was a tumor-specific event because it did not occur in the rats switched to the folate and methyl-adequate diet after 36 weeks on deficient diet, which did not lead to hepatocarcinogenesis. miR-122 was also silent in a transplanted rat hepatoma. Extrapolation of this study to human primary HCCs revealed that miR-122 expression was significantly (P = 0.013) reduced in 10 out of 20 tumors compared to the pair-matched control tissues. These findings suggest that the downregulation of miR-122 is associated with hepatocarcinogenesis and could be a potential biomarker for liver cancers. |
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Authors:
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Huban Kutay; Shoumei Bai; Jharna Datta; Tasneem Motiwala; Igor Pogribny; Wendy Frankel; Samson T Jacob; Kalpana Ghoshal |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 99 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-02 Completed Date: 2007-06-11 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 671-8 Citation Subset: IM |
Copyright Information:
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2006 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinoma, Hepatocellular / genetics, metabolism* Choline Deficiency Diet Folic Acid Deficiency Gene Expression Profiling Gene Expression Regulation, Neoplastic* Humans Liver Neoplasms / genetics, metabolism* Male Methionine / deficiency MicroRNAs / genetics, metabolism* Microarray Analysis Rats Rats, Inbred F344 |
| Grant Support | |
ID/Acronym/Agency:
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CA86978/CA/NCI NIH HHS; R01 CA086978-01A2/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 63-68-3/Methionine |
| Comments/Corrections | |
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