Document Detail


Downregulation of leukotriene biosynthesis by thymoquinone attenuates airway inflammation in a mouse model of allergic asthma.
MedLine Citation:
PMID:  16624488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic airway inflammation is a key feature of bronchial asthma. Leukotrienes are potent inflammatory mediators that play a role in the pathophysiology of asthma, and their levels are elevated in the airways in response to allergen challenge. We examined the anti-inflammatory effect of thymoquinone (TQ), the active principle in the volatile oil of Nigella sativa seeds, on leukotriene (LT) biosynthesis in a mouse model of allergic asthma. Mice sensitized and challenged with ovalbumin (OVA) antigen had an increased amounts of leukotriene B4 and C4, Th2 cytokines, and eosinophils in bronchoalveolar lavage (BAL) fluid. In addition, there was also a marked increase in lung tissue eosinophilia and goblet cell numbers. Administration of TQ before OVA challenge inhibited 5-lipoxygenase, the main enzyme in leukotriene biosynthesis, expression by lung cells and significantly reduced the levels of LTB4 and LTC4. This was accompanied by a marked decrease in Th2 cytokines and BAL fluid and lung tissue eosinophilia, all of which are characteristics of airway inflammation. These results demonstrate the anti-inflammatory effect of TQ in experimental asthma.
Authors:
Mohamed El Gazzar; Rabab El Mezayen; Mark R Nicolls; John C Marecki; Stephen C Dreskin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-03-31
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1760     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-19     Completed Date:  2006-08-28     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1088-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate 5-Lipoxygenase / metabolism
Asthma / pathology*
Benzoquinones / chemistry*
Bronchoalveolar Lavage Fluid
Cytokines / metabolism
Disease Models, Animal
Down-Regulation*
Female
Hypersensitivity / pathology
Inflammation*
Leukotrienes / biosynthesis*
Lung / pathology
Male
Mice
Mice, Inbred BALB C
Nigella sativa / metabolism
Grant Support
ID/Acronym/Agency:
HL 51241/HL/NHLBI NIH HHS; R01 HL082662/HL/NHLBI NIH HHS; R01 HL082662-01A1/HL/NHLBI NIH HHS; R01 HL095686/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/Cytokines; 0/Leukotrienes; 490-91-5/thymoquinone; EC 1.13.11.34/Arachidonate 5-Lipoxygenase

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