| Downregulation of immunodetectable connexin43 and decreased gap junction size in the pathogenesis of chronic hibernation in the human left ventricle. | |
| | |
MedLine Citation:
|
PMID: 9495300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The regional wall motion impairment and predisposition to arrhythmias in human ventricular hibernation may plausibly result from abnormal intercellular propagation of the depolarizing wave front. This study investigated the hypothesis that altered patterns of expression of connexin43, the principal gap junctional protein responsible for passive conduction of the cardiac action potential, contribute to the pathogenesis of hibernation. METHODS AND RESULTS: Patients with poor ventricular function and severe coronary artery disease underwent thallium scanning and MRI to predict regions of normally perfused, reversibly ischemic, or hibernating myocardium. Twenty-one patients went on to coronary artery bypass graft surgery, during which biopsies representative of each of the above classes were taken. Hibernation was confirmed by improvement in segmental wall motion at reassessment 6 months after surgery. Connexin43 was studied by quantitative immunoconfocal laser scanning microscopy and PC image software. Analysis of en face projection views of intercalated disks revealed a significant reduction in relative connexin43 content per unit area in reversibly ischemic (76.7+/-34.6%, P<.001) and hibernating (67.4+/-24.3%, P<.001) tissue compared with normal (100+/-30.3%); ANOVA P<.001. The hibernating regions were further characterized by loss of the larger gap junctions normally seen at the disk periphery, reflected by a significant reduction in mean junctional plaque size in the hibernating tissues (69.5+/-20.8%) compared with reversibly ischemic (87.4+/-31.2%, P=.012) and normal (100+/-31.5%, P<.001) segments; ANOVA P<.001. CONCLUSIONS: These results indicate progressive reduction and disruption of connexin43 gap junctions in reversible ischemia and hibernation. Abnormal impulse propagation resulting from such changes may contribute to the electromechanical dysfunction associated with hibernation. |
| | |
Authors:
|
R R Kaprielian; M Gunning; E Dupont; M N Sheppard; S M Rothery; R Underwood; D J Pennell; K Fox; J Pepper; P A Poole-Wilson; N J Severs |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Circulation Volume: 97 ISSN: 0009-7322 ISO Abbreviation: Circulation Publication Date: 1998 Feb |
Date Detail:
|
Created Date: 1998-03-17 Completed Date: 1998-03-17 Revised Date: 2009-09-29 |
Medline Journal Info:
|
Nlm Unique ID: 0147763 Medline TA: Circulation Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 651-60 Citation Subset: AIM; IM |
Affiliation:
|
Cardiac Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, England. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Cell Communication* Connexin 43 / analysis, biosynthesis*, genetics Coronary Disease / complications*, physiopathology, surgery Female Follow-Up Studies Gap Junctions / physiology* Gene Expression Regulation* Heart Ventricles Humans Image Processing, Computer-Assisted Male Microscopy, Confocal Microscopy, Fluorescence Microscopy, Immunoelectron Middle Aged Myocardial Infarction / complications Myocardial Stunning / etiology*, genetics, physiopathology Ventricular Function, Left |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Connexin 43 |
| Comments/Corrections | |
Comment In:
|
Circulation. 1998 Feb 24;97(7):630-2
[PMID:
9495295
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of neoangiogenesis in ischemic myocardium by human growth factors: first clinical results ...
Next Document: Regular physical activity and coronary risk factors in Japanese men.