| Downregulation of connexin 43 in nasopharyngeal carcinoma cells is related to promoter methylation. | |
| | |
MedLine Citation:
|
PMID: 17306607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Down-regulation of Cx43 expression had been shown to occur in nasopharyngeal carcinoma cells. The present study was undertaken to estimate if methylation of the promoter region in Cx43 gene was responsible for the repression of Cx43 expression in the CNE-1 nasopharyngeal carcinoma cells. Calcein transfer and lucifer yellow transfer were detected to evaluate gap junction intercellular communication (GJIC) in CNE-1 cells. It was found that the control CNE-1 cells showed no fluorescent dye transfer. After treatment with DNA methyltransferase inhibitor 5-aza-CdR, fluorescent dye transfer between cells became obvious. RT-PCR and Western blot were performed to determine the expression of Cx43 gene. The control CNE-1 cells showed a low expression level of Cx43, whereas 5-aza-CdR-treated CNE-1 cells showed an enhanced level of Cx43 expression. Methylation-sensitive restriction enzyme and PCR analysis showed that the methylation of the Cx43 gene promoter region occurred in CNE-1 cells. In addition, treatment with 5-aza-CdR inhibited the growth (including anchorage-independent growth) of CNE-1 cells, and resulted in an accumulation of cells in G0/G1 phase. These results indicate the promoter methylation as an important role in inactivation of Cx43 in CNE-1 cells. |
| | |
Authors:
|
Zong-Chun Yi; Hong Wang; Guang-Yao Zhang; Bing Xia |
Related Documents
:
|
10200417 - Selective inhibition of gap junction channel activity by synthetic peptides. 9151687 - Connexin46 is retained as monomers in a trans-golgi compartment of osteoblastic cells. 15131437 - The functional significance of cell clusters in the notochordal nucleus pulposus: survi... 21141677 - Osmotic loading of in situ chondrocytes in their native environment. 21965717 - A rapid imageable in vivo metastasis assay for circulating tumor cells. 16282447 - Endocytosis and a low-ph step are required for productive entry of equine infectious an... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-15 |
Journal Detail:
|
Title: Oral oncology Volume: 43 ISSN: 1368-8375 ISO Abbreviation: Oral Oncol. Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-09-11 Completed Date: 2008-04-03 Revised Date: 2013-06-03 |
Medline Journal Info:
|
Nlm Unique ID: 9709118 Medline TA: Oral Oncol Country: England |
Other Details:
|
Languages: eng Pagination: 898-904 Citation Subset: IM |
Affiliation:
|
Department of Biological Engineering, Beijing University of Aeronautics and Astronautics, 37 Xueyuan Road, Beijing 100083, PR China. yizc@buaa.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Azacitidine
/
analogs & derivatives,
pharmacology Carcinoma, Squamous Cell / genetics*, metabolism, pathology Cell Cycle Cell Line, Tumor Connexin 43 / genetics* DNA Methylation DNA Modification Methylases / antagonists & inhibitors Down-Regulation* Fluoresceins / metabolism Gap Junctions / metabolism Gene Expression Regulation, Neoplastic* Humans Isoquinolines / metabolism Nasopharyngeal Neoplasms / genetics*, metabolism, pathology Promoter Regions, Genetic* |
| Chemical | |
Reg. No./Substance:
|
0/Connexin 43; 0/Fluoresceins; 0/Isoquinolines; 320-67-2/Azacitidine; 776B62CQ27/decitabine; 77944-88-8/lucifer yellow; EC 2.1.1.-/DNA Modification Methylases; V0YM2B16TS/fluorexon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Squamous cell carcinoma of the superior gingival-buccal complex.
Next Document: Predictive value of histopathologic parameters in early squamous cell carcinoma of oral tongue.