Document Detail

Downregulation of connexin 43 in nasopharyngeal carcinoma cells is related to promoter methylation.
MedLine Citation:
PMID:  17306607     Owner:  NLM     Status:  MEDLINE    
Down-regulation of Cx43 expression had been shown to occur in nasopharyngeal carcinoma cells. The present study was undertaken to estimate if methylation of the promoter region in Cx43 gene was responsible for the repression of Cx43 expression in the CNE-1 nasopharyngeal carcinoma cells. Calcein transfer and lucifer yellow transfer were detected to evaluate gap junction intercellular communication (GJIC) in CNE-1 cells. It was found that the control CNE-1 cells showed no fluorescent dye transfer. After treatment with DNA methyltransferase inhibitor 5-aza-CdR, fluorescent dye transfer between cells became obvious. RT-PCR and Western blot were performed to determine the expression of Cx43 gene. The control CNE-1 cells showed a low expression level of Cx43, whereas 5-aza-CdR-treated CNE-1 cells showed an enhanced level of Cx43 expression. Methylation-sensitive restriction enzyme and PCR analysis showed that the methylation of the Cx43 gene promoter region occurred in CNE-1 cells. In addition, treatment with 5-aza-CdR inhibited the growth (including anchorage-independent growth) of CNE-1 cells, and resulted in an accumulation of cells in G0/G1 phase. These results indicate the promoter methylation as an important role in inactivation of Cx43 in CNE-1 cells.
Zong-Chun Yi; Hong Wang; Guang-Yao Zhang; Bing Xia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-15
Journal Detail:
Title:  Oral oncology     Volume:  43     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-11     Completed Date:  2008-04-03     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  898-904     Citation Subset:  IM    
Department of Biological Engineering, Beijing University of Aeronautics and Astronautics, 37 Xueyuan Road, Beijing 100083, PR China.
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MeSH Terms
Azacitidine / analogs & derivatives,  pharmacology
Carcinoma, Squamous Cell / genetics*,  metabolism,  pathology
Cell Cycle
Cell Line, Tumor
Connexin 43 / genetics*
DNA Methylation
DNA Modification Methylases / antagonists & inhibitors
Fluoresceins / metabolism
Gap Junctions / metabolism
Gene Expression Regulation, Neoplastic*
Isoquinolines / metabolism
Nasopharyngeal Neoplasms / genetics*,  metabolism,  pathology
Promoter Regions, Genetic*
Reg. No./Substance:
0/Connexin 43; 0/Fluoresceins; 0/Isoquinolines; 320-67-2/Azacitidine; 776B62CQ27/decitabine; 77944-88-8/lucifer yellow; EC 2.1.1.-/DNA Modification Methylases; V0YM2B16TS/fluorexon

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