| Downregulation of Rac1 activation by caffeic acid in aortic smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 15808886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Caffeic acid, a dietary phenol from coffee, fruits and vegetables, is an efficient antioxidant. However, little is known about its anti-oxidative mechanism in the modulation of fundamental cellular processes. In this study, we investigated whether caffeic acid regulates Rac1 GTPase activity, a partner of NADPH oxidase. Our results showed that caffeic acid decrease Rac1 protein level under basal conditions and incubation with angiotensin II (ANG II) in vascular smooth muscle cells. In a Rac-bound-to-PAK pull down assay, caffeic acid clearly inhibited Rac1 activity. We also observed that caffeic acid suppressed the generation of superoxide anion stimulated by ANG II that activates NADPH oxidase. On the other hand, co-incubation with caffei caid and cycloheximide significantly accelerated the Rac1 degradation. In addition, pretreatment with caffeic acid for 24 hours was able to prevent phosphorylation of MLC and HSP27, when cells were challenged with ANG II through the redox sensitive pathway. These results support the hypothesis that caffeic acid reduces Rac1 GTPase protein and activity level, followed by a down-regulation of NADPH oxidase activity. |
| | |
Authors:
|
Jin-Wen Xu; Katsumi Ikeda; Akira Kobayakawa; Takao Ikami; Yasuyo Kayano; Takahiko Mitani; Yukio Yamori |
Related Documents
:
|
17107826 - Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica... 15005146 - Construction of an electro-enzymatic bioreactor for the production of (r)-mandelate fro... 13996 - Synthesis of stereospecifically deuterated phenylalamines and determination of their co... 20703956 - Use of cellulase inhibitors to produce cellobiose. 15381006 - Structure of a heteroxylan of gum exudate of the palm scheelea phalerata (uricuri). 2308896 - Bioavailability improvement of mycophenolic acid through amino ester derivatization. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Life sciences Volume: 76 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2005 Apr |
Date Detail:
|
Created Date: 2005-04-05 Completed Date: 2005-05-18 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
|
Languages: eng Pagination: 2861-72 Citation Subset: IM |
Affiliation:
|
Frontier Health Science, School of Human Environmental Science, MUKOGAWA Women's University, Nishinomiya, Hyogo, 663-8179, Japan. jwxu@mwu.mukogawa-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Angiotensin II / metabolism Animals Antioxidants / pharmacology* Aorta / cytology* Blotting, Western Caffeic Acids / pharmacology* Cycloheximide / metabolism Enzyme Activation / drug effects HSP27 Heat-Shock Proteins Heat-Shock Proteins / metabolism Male Muscle, Smooth, Vascular / cytology, metabolism* Neoplasm Proteins / metabolism Phosphorylation / drug effects Protein-Serine-Threonine Kinases Rats Rats, Inbred WKY Superoxides / metabolism Transfection p21-Activated Kinases rac1 GTP-Binding Protein / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Caffeic Acids; 0/HSP27 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, rat; 0/Neoplasm Proteins; 11062-77-4/Superoxides; 11128-99-7/Angiotensin II; 331-39-5/caffeic acid; 66-81-9/Cycloheximide; EC 2.7.11.1/Pak1 protein, rat; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/p21-Activated Kinases; EC 3.6.1.-/Rac1 protein, rat; EC 3.6.5.2/rac1 GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of purified herbal extract of Salvia miltiorrhiza on ischemic rat myocardium after acute myo...
Next Document: The permeability transition pore as a pathway for the release of mitochondrial DNA.