| Downregulation of potassium chloride cotransporter KCC2 after transient focal cerebral ischemia. | |
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MedLine Citation:
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PMID: 20044519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: The potassium chloride cotransporter 2 (KCC2) is the main neuronal chloride extruder in the adult nervous system. Therefore, KCC2 is responsible for an inwardly directed electrochemical gradient of chloride that leads to hyperpolarizing GABA-mediated responses. Under some pathophysiological conditions, GABA has been reported to be depolarizing because of a downregulation of KCC2. This is the first study to our knowledge analyzing the expression of KCC2 after a focal cerebral ischemia. METHODS: Mild and severe ischemia were induced in rats by a transient occlusion of the middle cerebral artery for 30 and 120 minutes, respectively. KCC2 mRNA and protein expression were studied in the ischemic hemisphere after different reperfusion times (2 hour, 1 day, 7 days, 30 days, 168 days) by using quantitative polymerase chain reaction, Western blotting, and immunohistological staining. RESULTS: We found a substantial decrease of KCC2 mRNA and protein levels in the ischemic hemisphere, with a stronger downregulation of KCC2 after severe vs mild ischemia. Long-term surviving cells expressing KCC2 could be detected in the infarct core. These cells were identified as GABAergic interneurons mainly expressing parvalbumin. CONCLUSIONS: Our study revealed a substantial neuron-specific downregulation of KCC2 after focal cerebral ischemia. |
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Authors:
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Nadine Jaenisch; Otto W Witte; Christiane Frahm |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-31 |
Journal Detail:
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Title: Stroke; a journal of cerebral circulation Volume: 41 ISSN: 1524-4628 ISO Abbreviation: Stroke Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-23 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0235266 Medline TA: Stroke Country: United States |
Other Details:
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Languages: eng Pagination: e151-9 Citation Subset: IM |
Affiliation:
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Hans-Berger Clinic for Neurology, Friedrich-Schiller University, Erlanger Allee 101, 07747 Jena, Germany. christiane.frahm@med.uni-jena.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chlorides / metabolism* Down-Regulation / physiology* Ischemic Attack, Transient / metabolism*, pathology Male Potassium / metabolism* RNA, Messenger / metabolism Rats Rats, Wistar Symporters / antagonists & inhibitors*, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/RNA, Messenger; 0/Symporters; 0/potassium-chloride symporters; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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