| Downregulation of PLK-1 expression in kaempferol-induced apoptosis of MCF-7 cells. | |
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MedLine Citation:
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PMID: 19356725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecular mechanisms underlying the kaempferol-induced cell death have not yet been fully explained. To investigate the role of kaempferol, widely distributed in foods, in tumor progression, human breast cancer cell line, MCF-7, was treated with kaempferol. Apoptosis was indicated by the accumulation of a sub-G1 population, as well as the appearance of 4'-6-diamidino-2-phenylindole (DAPI)-stained apoptotic nuclei in the MCF-7 cells after the administration of kaempferol. Western blot analysis showed cleavage of Poly (ADP-ribose) polymerase (PARP), caspase-7, Bax, and caspase-9 indicating that the intracellular pathway of apoptosis was involved. Kaempferol also downregulated the expression of polo-like kinase 1 (PLK-1), which has been reported to regulate mitotic progression and to be upregulated in several human tumors. Taken together, these findings indicate that kaempferol-induced apoptosis by initiation of intrinsic caspase cascade and downregulation of PLK-1 expression. |
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Authors:
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Gum-Yong Kang; Eung-Ryoung Lee; Jung-Hyun Kim; Jin Woo Jung; Jun Lim; Sang Kyung Kim; Ssang-Goo Cho; Kwang Pyo Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-05 |
Journal Detail:
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Title: European journal of pharmacology Volume: 611 ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-11 Completed Date: 2009-07-23 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 17-21 Citation Subset: IM |
Affiliation:
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Institute of Biomedical Science and Technology, Department of Molecular Biotechnology, Konkuk University, Seoul, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 9 / metabolism Cell Cycle Proteins / genetics* Cell Line, Tumor Down-Regulation / drug effects* Enzyme Activation / drug effects Humans Intracellular Space / drug effects, metabolism Kaempferols / pharmacology* Protein-Serine-Threonine Kinases / genetics* Proto-Oncogene Proteins / genetics* Signal Transduction / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Kaempferols; 0/Proto-Oncogene Proteins; 520-18-3/kaempferol; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1; EC 3.4.22.-/Caspase 9 |
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