Document Detail


Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells.
MedLine Citation:
PMID:  17310218     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hybridoma cells display an increase in antibody productivity following exposure to hypertonic conditions. However, the underlying mechanism is not well understood. In the present study, we hypothesize that the nuclear factor of activated T cells 5 (NFAT5)/tonicity enhancer binding protein (TonEBP) functions to increase the antibody productivity of hybridoma cells. NFAT5 is an osmosensitive mammalian transcription factor. However, its ubiquitous expression in various organs that are not bathed in hypertonic milieu suggests that NFAT5 may also regulate cell growth and function under isotonic conditions. In this study, we examined the expression of NFAT5 in hybridoma cells by Western blot analysis, and found that it increased significantly in hypertonic medium. To further define the function of NFAT5 in hybridoma cells, RNA interference technique was used to downregulate the expression of NFAT5 in SGB-8 cells (a hybridoma cell line). In isotonic medium, antibody productivity of hybridoma cells was reduced by downregulation of NFAT5 while cell proliferation was not influenced. The results presented here demonstrate that NFAT5 not only plays an important role in osmotic stress response pathway in hybridoma cells but also is essential for optimal antibody productivity.
Authors:
Jihang Ju; Ke Zou; Hong Xie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell research     Volume:  17     ISSN:  1748-7838     ISO Abbreviation:  Cell Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  China    
Other Details:
Languages:  eng     Pagination:  264-70     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis*
Antibody Formation
Cell Proliferation / drug effects
Cells, Cultured
Down-Regulation / drug effects
Female
Hybridomas / metabolism*
Hypertonic Solutions / pharmacology
Mice
Mice, Inbred BALB C
RNA Interference*
Transcription Factors / antagonists & inhibitors,  metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Hypertonic Solutions; 0/Nfat5 protein, mouse; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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