Document Detail


Downregulation of LRIG2 expression by RNA interference inhibits glioblastoma cell (GL15) growth, causes cell cycle redistribution, increases cell apoptosis and enhances cell adhesion and invasion in vitro.
MedLine Citation:
PMID:  19421009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The leucine-rich and immunoglobulin-like domains (LRIG) gene family contains LRIG1, 2 and 3. LRIG1 is a negative regulator of EGFR, but little is known about the function of LRIG2. To determine the role of LRIG2 in the progression of glioma, we performed RNA interference-mediated knockdown of LRIG2 in a human glioma cell line (GL15). Downregulation of LRIG2 expression resulted in: rapid EGF-mediated loss of EGFR; decreased proliferation; G(0)/G(1) arrest; increased spontaneous apoptosis; enhanced cell adhesion and increased invasion capability of GL15 cells in vitro. These findings indicate that LRIG2 possesses distinct functions compared with LRIG1 and validate the attractiveness of LRIG2 as a target in glioma therapy.
Authors:
Baofeng Wang; Lin Han; Rudong Chen; Mingjun Cai; Fuxin Han; Ting Lei; Dongsheng Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-10
Journal Detail:
Title:  Cancer biology & therapy     Volume:  8     ISSN:  1555-8576     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-07-09     Completed Date:  2009-11-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1018-23     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics
Cell Adhesion / genetics
Cell Cycle / genetics
Cell Line, Tumor
Central Nervous System Neoplasms / genetics*,  pathology
Down-Regulation
Epidermal Growth Factor / pharmacology
Glioblastoma / genetics*,  pathology
Humans
Membrane Glycoproteins / biosynthesis,  deficiency,  genetics*
RNA Interference
RNA, Neoplasm / genetics
RNA, Small Interfering / genetics*
Receptor, Epidermal Growth Factor / biosynthesis,  genetics
Transfection
Chemical
Reg. No./Substance:
0/LRIG2 protein, human; 0/Membrane Glycoproteins; 0/RNA, Neoplasm; 0/RNA, Small Interfering; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor
Comments/Corrections
Comment In:
Cancer Biol Ther. 2009 Jun;8(11):1024-6   [PMID:  19430200 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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