| Downregulation of IL-13 gene transcription by T-bet in human T cells. | |
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MedLine Citation:
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PMID: 18504404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Downregulation of a Th2 cytokine, IL-4, by a Th1-specific transcription factor, T-bet, has been demonstrated. However, the regulatory role of T-bet in another Th2 cytokine, IL-13, is not fully delineated. METHODS: IL-13 mRNA expression in Jurkat cells was examined by quantitative RT-PCR, while the transcriptional activity of 5'-flanking region in the IL-13 gene encompassing -1077 to +49 was investigated by fluorescence-based promoter reporter assay. The effect of T-bet was investigated by transfection of the cells with the T-bet expression vector. RESULTS: Stimulation with phorbol ester plus Ca2+ ionophore clearly induced IL-13 gene transcription in Jurkat cells. Ectopically expressed T-bet significantly suppressed the inducible mRNA expression and promoter activity of IL-13. CONCLUSION: IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2-related diseases. |
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Authors:
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Kazuya Suzuki; Osamu Kaminuma; Takachika Hiroi; Fujiko Kitamura; Shoichiro Miyatake; Fumio Takaiwa; Hideki Tatsumi; Soichi Nemoto; Noriko Kitamura; Akio Mori |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-27 |
Journal Detail:
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Title: International archives of allergy and immunology Volume: 146 Suppl 1 ISSN: 1423-0097 ISO Abbreviation: Int. Arch. Allergy Immunol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-05-27 Completed Date: 2008-08-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 33-5 Citation Subset: IM |
Copyright Information:
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Copyright 2008 S. Karger AG, Basel. |
Affiliation:
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Department of Allergy and Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Down-Regulation / genetics Humans Interleukin-13 / genetics, metabolism* Ionophores / pharmacology Jurkat Cells Phorbol Esters / pharmacology Promoter Regions, Genetic RNA, Messenger / biosynthesis Reverse Transcriptase Polymerase Chain Reaction T-Box Domain Proteins / genetics, metabolism* T-Lymphocytes / drug effects, immunology* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-13; 0/Ionophores; 0/Phorbol Esters; 0/RNA, Messenger; 0/T-Box Domain Proteins; 0/T-box transcription factor TBX21; 7440-70-2/Calcium |
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