Document Detail


Downregulation of IL-13 gene transcription by T-bet in human T cells.
MedLine Citation:
PMID:  18504404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Downregulation of a Th2 cytokine, IL-4, by a Th1-specific transcription factor, T-bet, has been demonstrated. However, the regulatory role of T-bet in another Th2 cytokine, IL-13, is not fully delineated. METHODS: IL-13 mRNA expression in Jurkat cells was examined by quantitative RT-PCR, while the transcriptional activity of 5'-flanking region in the IL-13 gene encompassing -1077 to +49 was investigated by fluorescence-based promoter reporter assay. The effect of T-bet was investigated by transfection of the cells with the T-bet expression vector. RESULTS: Stimulation with phorbol ester plus Ca2+ ionophore clearly induced IL-13 gene transcription in Jurkat cells. Ectopically expressed T-bet significantly suppressed the inducible mRNA expression and promoter activity of IL-13. CONCLUSION: IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2-related diseases.
Authors:
Kazuya Suzuki; Osamu Kaminuma; Takachika Hiroi; Fujiko Kitamura; Shoichiro Miyatake; Fumio Takaiwa; Hideki Tatsumi; Soichi Nemoto; Noriko Kitamura; Akio Mori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-27
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  146 Suppl 1     ISSN:  1423-0097     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-27     Completed Date:  2008-08-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  33-5     Citation Subset:  IM    
Copyright Information:
Copyright 2008 S. Karger AG, Basel.
Affiliation:
Department of Allergy and Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Down-Regulation / genetics
Humans
Interleukin-13 / genetics,  metabolism*
Ionophores / pharmacology
Jurkat Cells
Phorbol Esters / pharmacology
Promoter Regions, Genetic
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
T-Box Domain Proteins / genetics,  metabolism*
T-Lymphocytes / drug effects,  immunology*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Interleukin-13; 0/Ionophores; 0/Phorbol Esters; 0/RNA, Messenger; 0/T-Box Domain Proteins; 0/T-box transcription factor TBX21; 7440-70-2/Calcium

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