| Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs. | |
| | |
MedLine Citation:
|
PMID: 23018532 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Telomere is a nucleoprotein structure at the ends of chromosomes that helps to protect the ends of chromosomes from being fused with other chromosomes. Knockout of histone methyltransferases Suv39h1 and Suv39h2 increases the telomere length in murine cells, whereas downregulation of SUV39H1 and SUV39H2 genes decreases the telomere length in human cells, suggesting that telomere biology is different among mammalian species. However, epigenetic regulation of the telomere has not been studied in mammals other than the human and mouse. In the present study, the effect of knockdown of SUV39H1 and SUV39H2 genes on telomere length was examined in porcine embryonic stem-like cells (pESLCs) and porcine embryonic fibroblasts (PEFs). The telomeres in SUV39H1 and SUV39H2 knockdown (SUV39KD) pESLCs (37.1 ± 0.9 kb) were longer (P<0.05) compared with those of the control (33.0 ± 0.7 kb). Similarly, SUV39KD PEFs had longer telomeres (22.1 ± 0.4 kb; P<0.05) compared with the control (17.8 ± 1.1 kb). Telomerase activities were not different between SUV39KD pESLCs (10.4 ± 1.7) and the control (10.1 ± 1.7) or between SUV39KD PEFs (1.0 ± 0.3) and the control (1.0 ± 0.4), suggesting that telomerase activities did not contribute to the telomere elongation in SUV39KD pESLCs and SUV39KD PEFs. Relative levels of trimethylation of histone H3 lysine 9 and expressions of DNMT1, DNMT3A and DNMT3B were decreased in SUV39KD cells, suggesting that telomere lengthening in SUV39KD pESLCs and SUV39KD PEFs might be not only related to the loss of histone modification marks but also linked to the decrease in DNA methyltransferase in pigs. |
| | |
Authors:
|
Thanh Quang Dang-Nguyen; Seiki Haraguchi; Tadashi Furusawa; Tamas Somfai; Masahiro Kaneda; Shinya Watanabe; Satoshi Akagi; Kazuhiro Kikuchi; Atsushi Tajima; Takashi Nagai |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-28 |
Journal Detail:
|
Title: The Journal of reproduction and development Volume: - ISSN: 1348-4400 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A Placebo-Controlled, Multiple Ascending Dose Study to Evaluate the Safety, Pharmacokinetics and Pha...
Next Document: [Medical ethics as professional ethics].