Document Detail


Down-regulation synaptic vesicle protein 2A in the anterior temporal neocortex of patients with intractable epilepsy.
MedLine Citation:
PMID:  19757204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synaptic vesicle protein 2A (SV2A) involvement has been reported in the animal models of epilepsy. The aim of this study was to investigate the expression of SV2A in human intractable epilepsy (IE) brain tissue. Using immunohistochemistry, immunofluorescence, and Western blot, we detected SV2A expression in tissue samples from the anterior temporal neocortex of 33 patients who had been surgically treated for IE. We compared these tissues with nine histologically normal anterior temporal lobe samples from controls. SV2A immunoreactive staining was 0.1651+/-0.0564 in patient group and 0.2347+/-0.0187 in the control group (p<0.05) using immunohistochemistry, and this finding was consistently observed with Western blot analysis (0.1727 +/- 0.0471 versus 0.3976+/-0.0983, p<0.05). Immunofluorescence staining showed that SV2A was mainly accumulated in neurons. Our findings demonstrate that down-regulation of SV2A is present in patients with temporal lobe epilepsy.
Authors:
Guibo Feng; Fei Xiao; Yang Lu; Zuchun Huang; Jie Yuan; Zheng Xiao; Zhiqin Xi; Xuefeng Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  39     ISSN:  1559-1166     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-14     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  354-9     Citation Subset:  IM    
Affiliation:
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Biological Markers / metabolism
Blotting, Western
Down-Regulation / physiology*
Epilepsy / metabolism*,  physiopathology
Female
Fluorescent Antibody Technique
Humans
Immunochemistry
Male
Membrane Glycoproteins / metabolism*
Middle Aged
Neocortex / metabolism*,  pathology,  physiopathology
Nerve Tissue Proteins / metabolism*
Neurons / metabolism,  pathology
Temporal Lobe / metabolism*,  pathology,  physiopathology
Young Adult
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Membrane Glycoproteins; 0/Nerve Tissue Proteins; 148845-93-6/SV2A protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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