Document Detail

Down regulation of ribosomal protein mRNAs during neuronal differentiation of human NTERA2 cells.
MedLine Citation:
PMID:  11100899     Owner:  NLM     Status:  MEDLINE    
We have analysed the expression of 32 ribosomal protein (RP) mRNAs during retinoic acid induced neuronal differentiation of human NTERA2 cells. Except for a new S27 variant (S27v), all were down regulated both in selectively replated differentiated neurons and the most differentiated continuous cultures, i.e., non-replated cultures. However, the expression profiles of the individual RP mRNAs were different, most (L3, L7, L8, L10, L13, L23a, L27a, L36a, L39, P0, S2, S3, S3a, S4X, S6, S9, S12, S13, S16, S19, S20, S23, and S27a) exhibited a constant down regulation, whereas a few were either initially constant (L11, L32, S8, and S11) or up regulated (L6, L15, L17, L31, and S27y) and then down regulated. The expression of S27v remained elevated in the most differentiated continuous cultures but was down regulated in replated differentiated neurons. The down regulation of RP mRNAs was variable: the expression levels in differentiated replated neurons were between 10% (S3) and 90% (S11) of the levels in undifferentiated cells. The ratio between rRNA and RP mRNA changed during the differentiation; in differentiated neurons there were, on average, about half the number of RP mRNAs per rRNA as compared to undifferentiated cells. The expression profiles of a few translation-related proteins were also determined. EF1alpha1, EF1beta1, and EF1delta were down regulated, whereas the expression of the neuron and muscle specific EF1alpha2 increased. The reduction in the expression of RP mRNAs was coordinated with a reduction in the expression level of the proliferation marker PCNA. The expression levels of most RP mRNAs were lower in purified differentiated post-mitotic neurons than in the most differentiated continuous cultures, despite similar levels of PCNA, suggesting that both the differentiation state and the proliferative status of the cells affect the expression of RP mRNAs.
M Bévort; H Leffers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  66     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-27     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  81-92     Citation Subset:  IM    
Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Cell Differentiation / physiology*
DNA Primers
Gene Expression Regulation*
Neurons / cytology*,  physiology
Polymerase Chain Reaction
RNA, Messenger / genetics
Ribosomal Proteins / genetics*
Transcription, Genetic*
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA Primers; 0/RNA, Messenger; 0/Ribosomal Proteins

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