Document Detail

Down-regulation of proteolytic complexes following EBV activation in BL cells.
MedLine Citation:
PMID:  17157806     Owner:  NLM     Status:  MEDLINE    
In Burkitt's lymphoma cells, Epstein Barr virus (EBV) latency products interact with the ubiquitin-proteasome system to promote episomal maintenance and immunological evasion while the tripeptidylpeptidase II (TPPII) functions as an alternative protease. In the present study, we have examined the activities and levels of the proteasome and TPPII complex in Raji and in Akata cells after induction of EBV lytic cycle. The results show that the chymotrypsin-like and caspase-like activities of the proteasome were substantially reduced in Raji and Akata cells. Similarly, TPPII activity was diminished in both cell lines but was recovered in Akata cells at longer time after induction. Protein levels of the alpha/beta subunits of the 20S proteasome and TPPII concentration decreased to different extents after EBV activation, whereas the ubiquitin binding S6' subunit of the 19S regulatory complex increased three to fourfold along with the levels of ubiquitin-conjugates. Collectively, these observations demonstrate impairment of two major cellular proteolytic systems at the onset of EBV lytic infection.
Giulia Matusali; Alessandra De Leo; Riccardo Gavioli; Laura Bertelli; Livia Di Renzo; Elena Mattia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  352     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-18     Completed Date:  2007-03-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  947-52     Citation Subset:  IM    
Department of Public Health Sciences, University "La Sapienza", P. le A. Moro 5, 00185 Rome, Italy.
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MeSH Terms
Burkitt Lymphoma / enzymology*
Cell Line
Herpesvirus 4, Human / physiology*
Peptide Hydrolases / metabolism*
Virus Activation*
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases

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