Document Detail


Down-regulation of programmed cell death 5 by insulin-like growth factor 1 in osteoarthritis chondrocytes.
MedLine Citation:
PMID:  23322062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The aim of this study was to investigate the expression of insulin-like growth factor (IGF)-1 and programmed cell death 5 (PDCD5) in osteoarthritis chondrocytes, and to explore the potential correlation between them in the apoptosis process of osteoarthritis chondrocytes.
METHODS: Patients with knee osteoarthritis were placed into four categories according to radiological staging. The mRNA and protein levels of IGF-1 and PDCD5 in osteoarthritis chondrocytes were respectively detected by quantitative reverse transcriptase polymerase chain reaction (qPCR) and western blotting. In addition, IGF-1 and PDCD5 protein expression in chondrocytes were also measured by immunohistochemistry. Apoptotic cells were measured by TUNEL staining.
RESULTS: Both the mRNA and protein levels of IGF-1 were down-regulated, while the levels of PDCD5 were up-regulated, and the mRNA and protein levels of IGF-1 were negatively correlated with those of PDCD5, respectively. The apoptotic cell was significantly increased in osteoarthritis chondrocytes compared with control. Importantly, the apoptosis rate was positively correlated with PDCD5 protein expression and negatively correlated with IGF-1 protein expression
CONCLUSIONS: We concluded that IGF-1 may down-regulate the expression of PDCD5 and thus inhibit the apoptosis of osteoarthritis chondrocytes.
Authors:
Chengqing Yi; Chunhui Ma; Zongping Xie; Guoqiao Zhang; Wangsheng Song; Xiaokai Zhou; Yun Cao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  International orthopaedics     Volume:  37     ISSN:  1432-5195     ISO Abbreviation:  Int Orthop     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-22     Completed Date:  2013-11-14     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  937-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Apoptosis Regulatory Proteins / genetics*,  metabolism
Blotting, Western
Cells, Cultured
Chondrocytes / drug effects*,  metabolism,  pathology
Down-Regulation / drug effects*
Humans
Insulin-Like Growth Factor I / genetics,  pharmacology*
Neoplasm Proteins / genetics*,  metabolism
Osteoarthritis, Knee / genetics*,  metabolism,  surgery
RNA, Messenger / metabolism
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Neoplasm Proteins; 0/PDCD5 protein, human; 0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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