| Down-regulation of integrin alpha M beta 2 ligand-binding function by the urokinase-type plasminogen activator receptor. | |
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MedLine Citation:
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PMID: 16905120 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell adhesion molecule integrin alphaMbeta2 associates with the urokinase-type plasminogen activator receptor (uPAR) on monocytes and neutrophils. uPAR also associates with members of the beta1 and beta3 integrins, and it modulates the ligand-binding function of these integrins. In this study, we showed that co-expressing uPAR with alphaMbeta2 in 293 transfectants down-regulated the ligand-binding capacity of alphaMbeta2 to denatured protein, fibrinogen, and intercellular adhesion molecule 1 (ICAM-1). Migration of transfectants on fibrinogen mediated by alphaMbeta2 was reduced in the presence of uPAR. In addition, the constitutive ligand-binding property of an alphaMbeta2 mutant was attenuated by its association with uPAR. Co-immunoprecipitation analyses using a panel of alphaMbeta2-specific mAbs suggest shielding of the ligand-recognition site of alphaMbeta2 by uPAR. |
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Authors:
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Man-Li Tang; Le-Sheng Kong; S K Alex Law; Suet-Mien Tan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-07 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 348 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-22 Completed Date: 2006-10-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1184-93 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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metabolism Cell Line Cell Movement / genetics Down-Regulation* / genetics Humans Ligands Macrophage-1 Antigen / immunology, metabolism* Protein Binding / genetics Receptors, Cell Surface / biosynthesis, genetics, physiology* Receptors, Urokinase Plasminogen Activator Transfection Urokinase-Type Plasminogen Activator / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Ligands; 0/Macrophage-1 Antigen; 0/PLAUR protein, human; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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