Document Detail


Down-regulation of integrin alpha M beta 2 ligand-binding function by the urokinase-type plasminogen activator receptor.
MedLine Citation:
PMID:  16905120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell adhesion molecule integrin alphaMbeta2 associates with the urokinase-type plasminogen activator receptor (uPAR) on monocytes and neutrophils. uPAR also associates with members of the beta1 and beta3 integrins, and it modulates the ligand-binding function of these integrins. In this study, we showed that co-expressing uPAR with alphaMbeta2 in 293 transfectants down-regulated the ligand-binding capacity of alphaMbeta2 to denatured protein, fibrinogen, and intercellular adhesion molecule 1 (ICAM-1). Migration of transfectants on fibrinogen mediated by alphaMbeta2 was reduced in the presence of uPAR. In addition, the constitutive ligand-binding property of an alphaMbeta2 mutant was attenuated by its association with uPAR. Co-immunoprecipitation analyses using a panel of alphaMbeta2-specific mAbs suggest shielding of the ligand-recognition site of alphaMbeta2 by uPAR.
Authors:
Man-Li Tang; Le-Sheng Kong; S K Alex Law; Suet-Mien Tan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-07
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  348     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-22     Completed Date:  2006-10-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1184-93     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / metabolism
Cell Line
Cell Movement / genetics
Down-Regulation* / genetics
Humans
Ligands
Macrophage-1 Antigen / immunology,  metabolism*
Protein Binding / genetics
Receptors, Cell Surface / biosynthesis,  genetics,  physiology*
Receptors, Urokinase Plasminogen Activator
Transfection
Urokinase-Type Plasminogen Activator / metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ligands; 0/Macrophage-1 Antigen; 0/PLAUR protein, human; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

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