| Down-regulation of gephyrin in temporal lobe epilepsy neurons in humans and a rat model. | |
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MedLine Citation:
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PMID: 21404332 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Gephyrin, which is a postsynaptic scaffolding protein participated in clustering GABA(A) receptors at inhibitory synapses, has been reported to be involved in temporal lobe epilepsy (TLE) recently. Here, we investigate gephyrin protein expression in the temporal lobe epileptic foci in epileptic patients and experimental animals in order to explore the probable relationship between gephyrin expression and TLE. Using immunohistochemistry, immunofluorescence, and western blot analysis, gephyrin expression was examined in thirty human temporal neocortex samples from patients who underwent surgery to treat drug-refractory TLE and ten histological normal temporal neocortex from the controls. Meanwhile, we investigated the gephyrin expression in the hippocampus and adjacent neocortex from experimental rats on 24 hours, 48 hours, 1 week, 2 weeks, 1 month, and 2 months post-seizure, and from control rats. Gephyrin protein was mainly expressed in the membrane and cytoplasm of neurons in temporal lobe epileptic foci in humans and experimental rats. Gephyrin expression was significantly lower in the temporal neocortex of TLE patients compared with the controls. In experimental rats, the expression of gephyrin in temporal lobe was down-regulated in epileptic groups compared to the control group. Gephyrin expression gradually decreased during the acute period and the latent period, but then began to increase below the levels seen in controls during the chronic phase. Our findings suggest that gephyrin may be involved in the development of TLE. Synapse, 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Min Fang; Lan Shen; Huan Yin; Yu-Min Pan; Liang Wang; Dan Chen; Zhi-Qin Xi; Zheng Xiao; Xue-Feng Wang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-14 |
Journal Detail:
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Title: Synapse (New York, N.Y.) Volume: - ISSN: 1098-2396 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806914 Medline TA: Synapse Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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