| Down-regulation of the expression of O-acetyl-GD3 by the O-acetylesterase cDNA in hamster melanoma cells: effects on cellular proliferation, differentiation, and melanogenesis. | |
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MedLine Citation:
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PMID: 10037466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The composition of the gangliosides of hamster melanoma cells is closely related to their cellular growth and degree of differentiation, with slow-growing, highly differentiated melanotic melanoma MI cells expressing GM3 and fast-growing, undifferentiated amelanotic Ab melanoma cells having a preponderance of GD3 and O-acetyl-GD3. To study the putative function of O-acetyl-GD3, we established stably transfected AbC-1 amelanotic hamster melanoma cells with O-acetylesterase gene from influenza C virus to hydrolyze the O-acetyl group from O-acetyl-GD3. The content of O-acetyl-GD3 in the transfected cells expressing O-acetylesterase gene was reduced by >90%. These O-acetyl-GD3-depleted cells differed from the parental ones in their cellular morphology, growth behavior, and melanogenesis activity. The absence of O-acetyl-GD3 in the transfected cells was accompanied by increased thick dendrite formation with an enlarged cell body, which is in striking contrast to the control cells, which were rounded and flattened, with few processes. Their growth was significantly slower than that of the control cells. They also demonstrated significantly lower tyrosinase activity and melanogenic potential. We suggest that the enhanced expression of melanoma-associated O-acetyl-GD3 ganglioside may stimulate cellular growth and suppress certain differentiated phenotypes such as dendrite formation but not melanogenesis. |
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Authors:
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S Birklé; S Ren; A Slominski; G Zeng; L Gao; R K Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 72 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-03-18 Completed Date: 1999-03-18 Revised Date: 2010-08-12 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 954-61 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biophysics, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond 23298-0614, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carboxylic Ester Hydrolases / biosynthesis*, genetics Cell Differentiation / genetics, physiology Cell Division / genetics, physiology Cricetinae DNA, Complementary / biosynthesis, genetics, physiology* Dendrites / metabolism, ultrastructure Down-Regulation* Enzyme-Linked Immunosorbent Assay Gangliosides / biosynthesis*, isolation & purification, metabolism Humans Immunohistochemistry Melanins / biosynthesis*, genetics Melanoma, Experimental / enzymology, genetics, metabolism*, pathology Plasmids Thymidine / metabolism Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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NS11853-24/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Gangliosides; 0/Melanins; 50-89-5/Thymidine; 98743-26-1/9-O-acetyl-GD3 ganglioside; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.6/sialate O-acetylesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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