| Down-regulation of c-Myc expression inhibits the invasion of bile duct carcinoma cells. | |
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MedLine Citation:
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PMID: 21557726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c-Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c-Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down-regulated c-Myc expression can inhibit tumour cell invasion still remains to be explored. The c-Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c-Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c-Myc ASODN could significantly suppress the c-Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c-Myc ASODN compared with that in the control and c-Myc NSODN-transfected group. Thus in the present study we show that down-regulation of c-Myc expression can inhibit the invasion of QBC939 cells in vitro. |
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Authors:
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Zhuo-Ri Li; Yi-Fei Wu; Chun-Yang Ma; Shen-Dan Nie; Xian-Hai Mao; Yong-Zhong Shi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-13 Completed Date: 2011-12-22 Revised Date: 2012-08-02 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 799-802 Citation Subset: IM |
Affiliation:
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Department of Hepatobiliary Surgery, Hainan Provincial Peoples Hospital, Haikou 570311, Peoples Republic of China. LZR59@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile Duct Neoplasms
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genetics,
metabolism*,
pathology Bile Ducts, Intrahepatic / pathology* Cell Line, Tumor Cell Proliferation / drug effects Cholangiocarcinoma / genetics, metabolism*, pathology Codon, Nonsense / genetics, pharmacology Down-Regulation / drug effects Gene Expression Regulation, Neoplastic Humans Liver / pathology Liver Neoplasms / metabolism Neoplasm Invasiveness Oligodeoxyribonucleotides, Antisense / genetics, pharmacology Proto-Oncogene Proteins c-myc / biosynthesis*, genetics Transfection |
| Chemical | |
Reg. No./Substance:
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0/Codon, Nonsense; 0/MYC protein, human; 0/Oligodeoxyribonucleotides, Antisense; 0/Proto-Oncogene Proteins c-myc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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