Document Detail


Down-regulation of apoptosis after left ventricular aneurysm repair.
MedLine Citation:
PMID:  17888983     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Apoptosis is a common feature of the cardiomyopathic process contributing to progressive decline in ventricular function after transmural myocardial infarction. We hypothesized that left ventricular aneurysm repair (LVAR) down-regulates apoptotic potential of cardiomyocytes in the surviving myocardium. METHODS: In the rat infarct model, LV aneurysms were repaired by pursestring suture 2 weeks after coronary artery ligation. Cardiac function and myocardial infarction size were assessed by echocardiography and transverse heart sections, respectively, before sacrifice 12 weeks later. Cardiomyocytes and TdT-mediated dUTP terminal nick-end labeling (TUNEL) assays of apoptotic nuclei were analyzed adjacent to and remote from the aneurysm (8 in infarction group, 7 in aneurysm group, and 11 in repair group). Biochemical samples for immunoblot were also obtained from surviving myocardium. RESULTS: A statistically significant increase in apoptotic rate was seen in both adjacent and remote areas (p < 0.01) after aneurysm formation. After LVAR, heart function was improved, and TUNEL assays also show significant decrease when compared with aneurysm group. But significant decreases were noted only in activated caspase-9 and increases in Bcl-2 in immunoblot analysis when comparing repair group with aneurysm group. CONCLUSIONS: Down-regulation of apoptosis accounts for the change in the long-term benefit after LVAR. To prevent heart failure, LVAR is indicated when it is large enough, and the infarction area should be excluded as much as possible.
Authors:
Chiao-Po Hsu; Chun-Yao Huang; Jih-Shiuan Wang; Hsin-I Chiang; Chun-Che Shih
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  84     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-24     Completed Date:  2007-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1279-87     Citation Subset:  AIM; IM    
Affiliation:
Division of Cardiovascular Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Apoptosis / physiology*
Disease Models, Animal
Down-Regulation*
Female
Heart Aneurysm / etiology,  pathology,  surgery*
Heart Failure / prevention & control
Heart Ventricles / pathology,  surgery
Immunoblotting
Immunohistochemistry
In Situ Nick-End Labeling
Myocardial Infarction / complications
Myocytes, Cardiac / pathology,  physiology*
Random Allocation
Rats
Rats, Sprague-Dawley
Reference Values
Comments/Corrections
Comment In:
Ann Thorac Surg. 2007 Oct;84(4):1287   [PMID:  17888984 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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