Document Detail


Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction.
MedLine Citation:
PMID:  15725075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand-receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB-EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells.
Authors:
Jurgen A Riedl; Dominique T Brandt; Eduard Batlle; Leo S Price; Hans Clevers; Johannes L Bos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  389     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-12-21     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  465-9     Citation Subset:  IM    
Affiliation:
Department of Physiological Chemistry, Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cell Shape
Colon / metabolism,  pathology
Down-Regulation*
Ephrin-B1 / metabolism*
Gene Expression Regulation
Humans
Receptor, EphB2 / metabolism
rap1 GTP-Binding Proteins / genetics,  metabolism*
ras GTPase-Activating Proteins / metabolism
rho GTP-Binding Proteins / metabolism
Chemical
Reg. No./Substance:
0/Ephrin-B1; 0/ras GTPase-Activating Proteins; EC 2.7.10.1/Receptor, EphB2; EC 3.6.5.2/rap1 GTP-Binding Proteins; EC 3.6.5.2/rho GTP-Binding Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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