Document Detail

Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients.
MedLine Citation:
PMID:  21575935     Owner:  NLM     Status:  Publisher    
OBJECTIVE: To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients. DESIGN: DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting. SETTING: Academic medical center and research laboratories. PATIENT(S): Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): DJ-1 in spermatozoa was determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit. RESULT(S): The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients. CONCLUSION(S): DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis.
Chun-Na An; Hui Jiang; Qi Wang; Ren-Pei Yuan; Jian-Ming Liu; Wan-Lu Shi; Zi-Yu Zhang; Xiao-Ping Pu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-14
Journal Detail:
Title:  Fertility and sterility     Volume:  -     ISSN:  1556-5653     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, People's Republic of China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science, Peking University, People's Republic of China.
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