| Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients. | |
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MedLine Citation:
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PMID: 21575935 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients. DESIGN: DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting. SETTING: Academic medical center and research laboratories. PATIENT(S): Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): DJ-1 in spermatozoa was determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit. RESULT(S): The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients. CONCLUSION(S): DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis. |
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Authors:
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Chun-Na An; Hui Jiang; Qi Wang; Ren-Pei Yuan; Jian-Ming Liu; Wan-Lu Shi; Zi-Yu Zhang; Xiao-Ping Pu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-14 |
Journal Detail:
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Title: Fertility and sterility Volume: - ISSN: 1556-5653 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, People's Republic of China; Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science, Peking University, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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