Document Detail


Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats.
MedLine Citation:
PMID:  23148924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the expression of CatSper1 channel in epididymal spermatozoa in a rat model of asthenozoospermia, induced by cyclophosphamide (CP), and further examine the effects of soluble granules of Sheng-Jing-San (SJS), a traditional Chinese medicine recipe, on CatSper1 expression and sperm motility in the CP-induced asthenozoospermic rats.
DESIGN: Placebo-controlled, randomized trial.
SETTING: Neuroscience Research Institute, Peking University, China.
ANIMAL(S): Sexually mature male Sprague-Dawley rats (n = 60).
INTERVENTION(S): In the CP group, CP at the dose of 35 mg/kg intraperitoneally injected into rats once a day for 7 days; in the normal saline (NS) group, 0.9% saline solution was injected as control.
MAIN OUTCOME MEASURE(S): Sperm motility and count were evaluated by computer-assisted sperm assay (CASA); protein and mRNA expression of CatSper1 channel in epididymal spermatozoa was determined by Western blotting and quantitative real-time RT-PCR, respectively.
RESULT(S): The rats were randomly divided into five groups with 12 rats in each group: CP, normal saline (NS), CP + SJS, CP + NS, and treatment naïve. In the CP + SJS group, after the last injection of CP, SJS at a dose of 30 mg/kg was intragastrically administrated to rats once a day for 14 days; in CP + NS group, saline solution instead of SJS was administrated as control. In the treatment naïve group, rats were normally fed for 21 days as controls. We found a statistically significant reduction of the CatSper1 channel, which is associated with an impairment of sperm motility in the epididymal spermatozoa of CP-induced asthenozoospermic rats. Soluble granules of SJS could dramatically restore the CP-induced down-regulation of CatSper1 in epididymal spermatozoa, which greatly improved the sperm motility in the asthenozoospermic rats.
CONCLUSION(S): Down-regulation of the CatSper1 channel in epididymal spermatozoa likely contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by SJS improves sperm motility and thus can be used as an effective therapeutic strategy for the treatment of male infertility diagnosed with asthenozoospermia.
Authors:
Ya-Nan Wang; Bo Wang; Ming Liang; Cai-Yan Han; Bin Zhang; Jie Cai; Wei Sun; Guo-Gang Xing
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2012-11-10
Journal Detail:
Title:  Fertility and sterility     Volume:  99     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-04     Completed Date:  2013-04-04     Revised Date:  2013-10-24    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  579-87     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
Neuroscience Research Institute and Department of Neurobiology, Peking University, Beijing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Asthenozoospermia / drug therapy*,  metabolism*
Calcium Channels / metabolism*
Down-Regulation
Drugs, Chinese Herbal / pharmacology*
Epididymis / cytology,  drug effects,  metabolism*
Gene Expression Regulation / drug effects
Male
Rats
Rats, Sprague-Dawley
Sperm Motility / drug effects*,  physiology
Spermatozoa / drug effects,  physiology
Treatment Outcome
Up-Regulation
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/CatSper1 protein, rat; 0/Drugs, Chinese Herbal; 0/sheng-ji-san
Comments/Corrections
Comment In:
J Urol. 2013 Oct;190(4):1436-7   [PMID:  24029361 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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