| Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates. | |
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MedLine Citation:
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PMID: 22510518 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Myosin light-chain kinase (MLCK) is a multi-domain protein with kinase and actin-binding domains, among others. Deficiency of MLCK expression in GBaSM-4 vascular smooth muscle cells enhanced cell proliferation rate and shortened cell doubling time. Transient transfection of the MLCK-deficient cells with cDNA constructs of either wild-type MLCK or its mutant lacking the kinase activity reverted the cell proliferation rate to that of wild-type cells, whereas that of MLCK lacking the actin-binding domain maintained cell proliferation at an elevated rate similar to the MLCK-deficient cells. Thus, the actin-binding domain of MLCK seems to play a role in regulating cell proliferation. |
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Authors:
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Hong Hui Wang; Akio Nakamura; Shinji Yoshiyama; Ryoki Ishikawa; Na Cai; Li-Hong Ye; Hiromi Takano-Ohmuro; Kazuhiro Kohama |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-18 |
Journal Detail:
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Title: Journal of pharmacological sciences Volume: - ISSN: 1347-8648 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167001 Medline TA: J Pharmacol Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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