Document Detail

Doublecortin-expressing cells in the ischemic penumbra of a small-vessel stroke.
MedLine Citation:
PMID:  17960829     Owner:  NLM     Status:  MEDLINE    
A cortical lesion was induced by disrupting the medium-size pial vessels, which led to a cone-shaped cortical lesion and turned into a fluid-filled cavity surrounded by a glial acidic fibrillary protein-positive (GFAP(+)) glia limitans 21 days after injury. Therefore, it mimics conditions of lacunar infarctions, one of the most frequent human stroke pathologies. Doublecortin (DCX)-positive cells were present in the neocortex and corpus callosum at the base of the lesion. The number of DCX-positive cells in the corpus callosum was significantly increased from day 5 to day 14 compared with the control group. In contrast, there were no DCX-positive cells in neocortex of control animals; the DCX-positive cells appeared in the neocortex after lesioning and were maintained until 14 days postlesioning. Some of the DCX-positive cells were also immunoreactive for beta III-tubulin, another marker of immature neurons. They did not stain positively for markers of glia cells. The presence of these DCX-positive cells near the lesion might indicate a migratory pathway for developing neuroblasts from the subventricular zone (SVZ) through the corpus callosum to the lesion. SVZ cells were labeled with a lipophilic molecule, 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE) stereotaxical injections. Although rostral migratory stream and olfactory bulb were intensely labeled, no CFSE-containing cells were found in the cortex beneath the lesion. These results do not support the idea that the DCX-positive cells were originating from neural precursors of the SVZ, but they might be generated from local progenitor cells.
Rui Hua; Ron Doucette; Wolfgang Walz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  86     ISSN:  1097-4547     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  883-93     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
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MeSH Terms
Brain Infarction / etiology,  metabolism,  pathology
Cell Differentiation
Cell Movement / physiology
Image Processing, Computer-Assisted
Microscopy, Confocal
Microtubule-Associated Proteins / biosynthesis*
Neurons / cytology,  metabolism*
Neuropeptides / biosynthesis*
Pia Mater / blood supply
Rats, Wistar
Stem Cells / cytology,  metabolism*
Stroke / complications,  metabolism*,  pathology
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/Neuropeptides; 0/doublecortin protein

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