| Double-strand hydrolysis of DNA by a magnesium(II) complex with diethylenetriamine. | |
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MedLine Citation:
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PMID: 15112075 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of artificial nucleases that hydrolyze DNA or RNA is of great interest in molecular biology, biotechnology, and medicine. We now report that a magnesium(II) complex of diethylenetriamine (Mg-dien) can effectively promote the double-stranded cleavage of plasmid DNA and the dideoxynucleotide dApdA under physiological conditions of pH and temperature. Experiments performed in the presence of hydrogen peroxide, radical scavengers, or under rigorously anaerobic conditions indicate that DNA cleavage mediated by Mg-dien occurs via a hydrolytic path. Mg-dien efficiently hydrolyzes supercoiled pBR322 DNA and the pseudo-first-order rate constant at 37 degrees C and pH 8.0 is estimated to be 1.60 h(-1). The dinucleotide dApdA hydrolysis, with Mg-dien at 170 microM, shows a rate enhancement factor of ca. 5 x 10(8). 1H and 31P(1H) NMR studies show that Mg-dien effectively hydrolyzes 5'-dAMP to give deoxyadenosine and inorganic phosphate. While Mg2+ has been found at the catalytic sites of many natural nucleases, Mg-dien appears to be the first synthetic Mg2+-containing system capable of hydrolyzing dideoxynucleotides and DNA and thus may provide a simple model system to assist mechanistic studies of naturally occurring nucleases. |
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Authors:
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Pin Yang; Rui Ren; Maolin Guo; Aixin Song; Xiangli Meng; Caixia Yuan; Qinghua Zhou; Huili Chen; Zhenhai Xiong; Xiaoli Gao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-04-27 |
Journal Detail:
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Title: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry Volume: 9 ISSN: 0949-8257 ISO Abbreviation: J. Biol. Inorg. Chem. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-01 Completed Date: 2005-03-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9616326 Medline TA: J Biol Inorg Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 495-506 Citation Subset: IM |
Copyright Information:
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Copyright 2004 SBIC |
Affiliation:
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Institute of Molecular Science, Shanxi University, 030006 Taiyuan, Shanxi Province, China. yangpin@sxu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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chemistry*,
metabolism Deoxyribonucleases / chemistry* Dinucleoside Phosphates Hydrolysis Magnesium / chemistry* Molecular Mimicry Molecular Structure Plasmids Polyamines / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Dinucleoside Phosphates; 0/Polyamines; 111-40-0/diethylenetriamine; 7439-95-4/Magnesium; 9007-49-2/DNA; EC 3.1.-/Deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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