| Double in situ hybridization for simultaneous detection and differentiation of porcine circovirus 1 and 2 in pigs with postweaning multisystemic wasting syndrome. | |
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MedLine Citation:
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PMID: 12505399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Double in situ hybridization using a digoxigenin-labelled porcine circovirus 1 (PCV1) and biotinylated PCV2 probe, was developed for the simultaneous detection and differentiation of PCV1 and PCV2 in formalin-fixed, paraffin-embedded tissues from pigs with postweaning multisystemic wasting syndrome. The combination of an alkaline phosphatase conjugated antidigoxigenin system with alkaline phosphatase conjugated streptavidin-biotin system allowed identification of PCV1 and/or PCV2. No evidence of cross-reaction was observed. Positive cells exhibited a red or dark brown reaction product for PCV1 and PCV2, respectively. Both PCV DNAs were observed mainly in the cytoplasm but occasionally in the nucleus. Co-localization of hybridization signal for both PCV1 and PCV2 was present in macrophages and multinucleated giant cells of the lymph node and spleen. This double-labelling technique for the differentiation between PCV1 and PCV2 is suitable for pathogenesis studies and diagnostic applications. |
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Authors:
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J Kim; C Chae |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Veterinary journal (London, England : 1997) Volume: 164 ISSN: 1090-0233 ISO Abbreviation: Vet. J. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-12-30 Completed Date: 2003-05-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9706281 Medline TA: Vet J Country: England |
Other Details:
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Languages: eng Pagination: 247-53 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Elsevier Science Ltd. |
Affiliation:
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Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Circoviridae Infections / complications*, veterinary* Circovirus / classification*, genetics, isolation & purification* In Situ Hybridization Lymph Nodes / virology RNA, Viral / analysis*, genetics Spleen / virology Swine / virology Swine Diseases / virology* Wasting Syndrome / complications*, veterinary* Weaning |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
| Comments/Corrections | |
Comment In:
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Vet J. 2002 Nov;164(3):171-2
[PMID:
12505388
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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