Document Detail


Double fluorescent staining for the separate demonstration of chromosomes and microtubules in mitotic cells in vitro.
MedLine Citation:
PMID:  89722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple fluorescent method for double staining of mitotic cells using a rhodamine B indirect immunofluorescent method for tubulin and the DNA-specific fluorescent dye Hoechst 33258 for nuclei and chromosomes is described. This procedure enables one through the use of appropriate excitation filters to view at will either chromosomes and nuclei or tubulin within the same cell.
Authors:
B R Brinkley; S M Cox
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stain technology     Volume:  53     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1978 Nov 
Date Detail:
Created Date:  1979-10-24     Completed Date:  1979-10-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  345-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bisbenzimidazole / diagnostic use
Cell Line
Chromosomes / analysis*
DNA / analysis
Fluorescent Antibody Technique
Microtubules / analysis*
Mitosis*
Rhodamines / diagnostic use
Staining and Labeling*
Tubulin / analysis
Chemical
Reg. No./Substance:
0/Rhodamines; 0/Tubulin; 23491-44-3/Bisbenzimidazole; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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