Document Detail


Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the diagnosis of schistosomiasis.
MedLine Citation:
PMID:  2794579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a dot enzyme-linked immunosorbent assay (dot-ELISA) as a field applicable tool for the rapid diagnosis of schistosomiasis. This antibody capture assay is performed using 50 ng protein of crude Schistosoma mansoni egg antigen (SEA) in 1 microliter volumes per dot. Sera (1 microliter/dot) at 1:40 dilution were optimal. Dot-ELISA results were completely comparable to micro-ELISA.
Authors:
H A Hamadto; K A Kamal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the Egyptian Society of Parasitology     Volume:  19     ISSN:  1110-0583     ISO Abbreviation:  J Egypt Soc Parasitol     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1989-11-09     Completed Date:  1989-11-09     Revised Date:  2013-05-02    
Medline Journal Info:
Nlm Unique ID:  8102141     Medline TA:  J Egypt Soc Parasitol     Country:  EGYPT    
Other Details:
Languages:  eng     Pagination:  921-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Animals
Antibodies, Helminth / analysis
Child
Enzyme-Linked Immunosorbent Assay
Humans
Immunoblotting
Male
Schistosoma / immunology
Schistosomiasis / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Helminth

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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