Document Detail


Dot-ELISA in diagnosis of schistosomiasis.
MedLine Citation:
PMID:  2794577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One microliter of S. mansoni egg antigen was dotted directly on the nitrocellulose paper sheet acting as the adsorbent surface (9 dots/paper). The sera of 25 Egyptian patients and 15 healthy persons (2 microliters of each) were dotted over the antigen dots, then 2 ml of each of the blocking, washing, HRP-conjugated IgG and DAB adding procedures, were added over the nitrocellulose paper in the petri-dish at room temperature. An intact brown circle (by naked-eye) indicates a positive in Dot-ELISA. There is an insignificant dot colour intensities in different clinical stages of S. mansoni infected Egyptians whereas, a direct relation was obtained between egg count and the colour intensity of the dots. The test had 100% sensitivity and 86% specificity thus it appears to be useful for both laboratory and field studies.
Authors:
M A Madwar; M M Hassan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the Egyptian Society of Parasitology     Volume:  19     ISSN:  1110-0583     ISO Abbreviation:  J Egypt Soc Parasitol     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1989-11-09     Completed Date:  1989-11-09     Revised Date:  2013-05-02    
Medline Journal Info:
Nlm Unique ID:  8102141     Medline TA:  J Egypt Soc Parasitol     Country:  EGYPT    
Other Details:
Languages:  eng     Pagination:  887-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Helminth / analysis
Enzyme-Linked Immunosorbent Assay
Humans
Immunoblotting
Schistosoma / immunology
Schistosomiasis / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Helminth

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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