Document Detail


Dose and time dependent apoptotic response in a human melanoma cell line exposed to accelerated boron ions at four different LET.
MedLine Citation:
PMID:  16019936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim was to investigate and compare the influence of linear energy transfer (LET), dose and time on the induction of apoptosis in a human melanoma cell line exposed to accelerated light boron ((10)B) ions and photons. Cells were exposed in vitro to doses up to 6 Gy accelerated boron ions (40, 80, 125 and 160 eV nm(-1)) and up to 12 Gy photons (0.2 eV nm(-1)). The induction of apoptosis was measured up to 9 days after irradiation using morphological characterization of apoptotic cells and bodies. In parallel, measurements of cell-cycle distribution, monitored by DNA flow cytometry, and cell survival based on the clonogenic cell survival assay, were performed. In addition, the induction and repair of DNA double-strand breaks (DSB), using pulsed-field gel electrophoresis (PFGE) were studied. Accelerated boron ions induced a significant increase in apoptosis as compared with photons at all time points studied. At 1-5 h the percentage of radiation-induced apoptotic cells increased with both dose and LET. At the later time points (24-216 h) the apoptotic response was more complex and did not increase in a strictly LET-dependent manner. The early premitotic apoptotic cells disappeared at 24 h following exposure to the highest LET (160 eV nm(-1)). A postmitotic apoptotic response was seen after release of the dose-, time- and LET-dependent G2/M accumulations. The loss of clonogenic ability was dose- and LET-dependent and the fraction of un-rejoined DSB increased with increasing LET. Despite the LET-dependent clonogenic cell killing, it was not possible to measure quantitatively a LET-dependent apoptotic response. This was due to the different time course of appearance and disappearance of apoptotic cells.
Authors:
A E Meijer; A R-M Jernberg; T Heiden; B Stenerlöw; L M Persson; N Tilly; B K Lind; M R Edgren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  81     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-07-15     Completed Date:  2005-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  261-72     Citation Subset:  IM; S    
Affiliation:
Department of Oncology-Pathology, Division of Medical Radiation Physics, Karolinska Institutet, Stockholm, Sweden. annelie.meijer@cck.ki.se
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Boron / therapeutic use*
Cell Division / radiation effects
Cell Line, Tumor
Cell Survival / radiation effects
DNA Damage
DNA Repair
G2 Phase / radiation effects
Humans
Linear Energy Transfer*
Melanoma / pathology,  radiotherapy*
Chemical
Reg. No./Substance:
7440-42-8/Boron

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