| Dose-dependent effects of gonadotropin on oocyte developmental competence and apoptosis. | |
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MedLine Citation:
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PMID: 22127004 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The aim of the present study was to evaluate the effect of gonadotropins (Gn) on oocyte maturation, developmental competence and apoptosis in an animal model. Bovine cumulus-oocyte complexes (COCs) were matured for 24h in media supplemented with varying concentrations of Bravelle (B), B+Menopur (B+M) or B+Repronex (B+R) (Ferring Pharmaceuticals, Parsiappany, NJ, USA). Then, nuclear maturation, embryo development, and apoptosis in cumulus cells and oocytes were evaluated. Low to moderate Gn concentrations (75-7500mIUmL(-1)) effectively improved nuclear maturation and in vitro development. Higher concentrations of Gn (75000mIUmL(-1)) did not have any added beneficial effects and nuclear maturation and blastocyst rates in the presence of these concentrations were comparable to control (P>0.05). Most COCs showed slight apoptosis when exposed to 75, 750 and 7500mIUmL(-1) Gn; however, when the concentration was increased to 75000mIUmL(-1), the proportion of moderately apoptotic COCs increased. In conclusion, extremely high concentrations of Gn have detrimental effects on oocyte nuclear maturation and embryo development and increase apoptosis in cumulus cells, suggesting the importance of judicious use of Gn in assisted reproductive technologies (ART). |
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Authors:
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Shan Liu; Huai L Feng; Dennis Marchesi; Zi-Jiang Chen; Avner Hershlag |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 23 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 990-6 Citation Subset: IM |
Affiliation:
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Center for Human Reproduction, North Shore-Long Island Jewish Health System, New York University School of Medicine, Manhasset, NY 11030, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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