Document Detail


Dose-dependent effects of dietary gamma-linolenic acid on rat spleen lymphocyte functions.
MedLine Citation:
PMID:  10477037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Feeding rodents a diet rich in evening primrose oil (EPO), which contains 5-10 g gamma-linolenic acid (GLA)/100 g total fatty acids, has been shown to decrease lymphocyte proliferation and natural killer cell activity. However, EPO contains a very high level of linoleic acid which itself can affect lymphocyte functions and it is not clear to what extent the effects of EPO can be attributed to GLA. The current study investigated the effect of two levels of GLA in the rat diet upon immune cell functions; the level of linoleic acid was maintained below 30 g/100 g total fatty acids. Weanling rats were fed on high fat (178 g/kg) diets which contained 4.4 g or 10 g GLA/100 g total fatty acids in place of a proportion of linoleic acid. The total polyunsaturated fatty acid content and the n-6 to n-3 polyunsaturated fatty acid ratio of the diet were maintained at 35 g/100 g total fatty acids and 7, respectively. The fatty acid compositions of the serum and of spleen leukocytes were markedly influenced by that of the diet, with an increase in the proportions of GLA and dihomo-gamma-linolenic acid when the diets containing GLA were fed; these diets also increased the proportion of arachidonic acid in spleen leukocytes. Spleen lymphocyte proliferation in response to concanavalin A was significantly reduced (by 60%) by feeding the diet containing the higher level of GLA, but not by the diet containing the lower level of GLA. Spleen natural killer cell activity and prostaglandin E (PGE) production by spleen leukocytes were not significantly affected by inclusion of GLA in the diet, although there was a tendency towards decreased natural killer cell activity by cells from rats fed the high GLA diet. Thus, this study shows that dietary GLA is capable of altering the fatty acid composition of cells of the immune system and of exerting some immunomodulatory effects, but that the level of GLA in the diet must exceed 4.4 g/100 g total fatty acids for these effects to become apparent.
Authors:
L D Peterson; F Thies; P C Calder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  61     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-10-07     Completed Date:  1999-10-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  19-24     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Oxford, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Concanavalin A / pharmacology
Dietary Fats, Unsaturated / administration & dosage*
Dose-Response Relationship, Drug
Fatty Acids / blood,  metabolism
Fatty Acids, Essential
Fatty Acids, Omega-3 / administration & dosage
Fatty Acids, Omega-6
Fatty Acids, Unsaturated / administration & dosage
Killer Cells, Natural / immunology
Linoleic Acids
Lymphocyte Activation / drug effects
Lymphocytes / immunology*,  metabolism
Male
Plant Oils
Prostaglandins E / biosynthesis
Rats
Rats, Inbred Lew
Spleen / cytology*
gamma-Linolenic Acid / administration & dosage*
Chemical
Reg. No./Substance:
0/Dietary Fats, Unsaturated; 0/Fatty Acids; 0/Fatty Acids, Essential; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Fatty Acids, Unsaturated; 0/Linoleic Acids; 0/Plant Oils; 0/Prostaglandins E; 11028-71-0/Concanavalin A; 506-26-3/gamma-Linolenic Acid; 65546-85-2/Efamol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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