Document Detail

Dose-dependent effect of EGF on migration and differentiation of adult subventricular zone astrocytes.
MedLine Citation:
PMID:  20187143     Owner:  NLM     Status:  MEDLINE    
Adult neural stem cells (NSCs) are located in the subventricular zone (SVZ), a specialized brain niche located on the walls of the lateral ventricle. Under physiological conditions, NSCs generate a large number of young neurons and some oligodendrocytes, however the mechanisms controlling cell proliferation and migration are unclear. In vitro, epidermal growth factor (EGF) signaling has been shown to be an important mediator of cell proliferation and migration in the adult brain; however, the primary SVZ progenitors that respond to EGF are not well known. In this study, we isolated SVZ type-B astrocytes and cultured them under different EGF concentrations. We found a dose-dependent effect of EGF on proliferation rates and migration of SVZ type-B astrocytes. We found that GFAP+ type-B astrocytes gave rise to highly migratory and proliferating cells that expressed Olig2 and NG2. After EGF withdrawal, a significant number of EGF-stimulated cells differentiated into S100beta+/O4+ oligodendrocytes. This study provides new insights about the production of oligodendrocytes derived from the astrocyte NSCs residing in the adult SVZ. To be able to manipulate the endogenous adult progenitors, it is crucial to identify and isolate the responding primary precursors and determine the extracellular signals that regulate their cell division, migration, and fate.
Oscar Gonzalez-Perez; Alfredo Quiñones-Hinojosa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  58     ISSN:  1098-1136     ISO Abbreviation:  Glia     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-04-22     Completed Date:  2010-07-12     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  975-83     Citation Subset:  IM    
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MeSH Terms
Adult Stem Cells / drug effects*
Antigens, CD24 / metabolism
Astrocytes / drug effects*
Basic Helix-Loop-Helix Transcription Factors / metabolism
Cell Differentiation / drug effects*
Cell Movement / drug effects*
Cells, Cultured
Dose-Response Relationship, Drug
Epidermal Growth Factor / pharmacology*
Glial Fibrillary Acidic Protein / metabolism
Lateral Ventricles / cytology*
Microtubule-Associated Proteins / metabolism
Nerve Tissue Proteins / metabolism
Neuropeptides / metabolism
Grant Support
K08NS055851/NS/NINDS NIH HHS; R01 NS070024/NS/NINDS NIH HHS; R01 NS070024-01/NS/NINDS NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Antigens, CD24; 0/Ascl1 protein, mouse; 0/Basic Helix-Loop-Helix Transcription Factors; 0/Glial Fibrillary Acidic Protein; 0/Microtubule-Associated Proteins; 0/Nerve Tissue Proteins; 0/Neuropeptides; 0/Olig2 protein, mouse; 0/doublecortin protein; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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