Document Detail

Dose-dependent functional benefit of human cardiosphere transplantation in mice with acute myocardial infarction.
MedLine Citation:
PMID:  22225626     Owner:  NLM     Status:  MEDLINE    
Despite mounting pre-clinical and clinical evidence of the beneficial effects of cell-based therapy, optimal cell dosing and delivery approaches have not been identified. Cardiospheres are self-assembling three-dimensional (3D) microtissues formed by cardiac stem cells and supporting cell types. The ability of cardiospheres to augment cardiac function has been demonstrated in animal models of ischemic cardiomyopathy. In this study, we studied the dose dependence of the benefits of human cardiospheres, delivered via intramyocardial injection, upon cardiac function and ventricular remodelling in SCID mice with acute myocardial infarction. Four doses of cardiospheres were used: 1 × 10(4), 5 × 10(4), 1 × 10(5) and 5 × 10(5) (expressed as number of plated cardiosphere-forming cells). Acute (24 hr) cell retention rates in all groups were similar. Functional assessment and quantitative heart morphometry indicated benefit from higher cell doses (≥5 × 10(4)) in terms of ejection fraction, infarct size and capillary density. Histological analysis indicated that the dose-dependent benefit was primarily because of indirect effects of transplanted cells. The results provide scalable data on cardiosphere dosing for intramyocardial injection.
Deliang Shen; Ke Cheng; Eduardo Marbán
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  16     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-23     Completed Date:  2013-01-07     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  2112-6     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
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MeSH Terms
Acute Disease
Mice, SCID
Myocardial Infarction / pathology,  therapy*
Myocardium / metabolism,  pathology*
Myocytes, Cardiac / cytology,  pathology,  transplantation*
Neovascularization, Physiologic
Spheroids, Cellular
Stem Cell Transplantation / methods*
Stem Cells / cytology,  metabolism
Ventricular Remodeling
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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