Document Detail

Doppler estimation of reduced coronary flow reserve in mice with pressure overload cardiac hypertrophy.
MedLine Citation:
PMID:  18255218     Owner:  NLM     Status:  MEDLINE    
Aortic banding produces pressure overload cardiac hypertrophy in mice, leading to decompensated heart failure in four to eight weeks, but the effects on coronary blood flow velocity and reserve are unknown. To determine whether coronary flow reserve (CFR) was reduced, we used noninvasive 20-MHz Doppler ultrasound to measure left main coronary flow velocity at baseline (B) and at hyperemia (H) induced by low (1%) and high (2.5%) concentrations of isoflurane gas anesthesia. Ten mice were studied before (Pre) and at 1 d, 7 d, 14 d and 21 d after constricting the aortic arch to 0.4 mm diameter distal to the innominate artery. We also measured cardiac inflow and outflow velocities at the mitral and aortic valves and velocity at the jet distal to the aortic constriction. The pressure drop as estimated by 4V2 at the jet was 51 +/- 5.1 (mean +/- SE) mm Hg at 1 d, increasing progressively to 74 +/- 5.2 mm Hg at 21 d. Aortic and mitral blood velocities were not significantly different after banding (p = NS), but CFR, as estimated by H/B, dropped progressively from 3.2 +/- 0.3 before banding to 2.2 +/- 0.4, 1.7 +/- 0.3, 1.4 +/- 0.2 and 1.1 +/- 0.1 at 1 d, 7 d, 14 d and 21 d, respectively (all p < 0.01 vs. Pre). There was also a significant and progressive increase the systolic/diastolic velocity ratio (0.17 Pre to 0.92 at 21 d, all p < 0.01 vs. Pre) suggesting a redistribution of perfusion from subendocardium to subepicardium. We show for the first time that CFR, as estimated by the hyperemic response to isoflurane and measured by Doppler ultrasound, can be measured serially in mice and conclude that CFR is virtually eliminated in banded mice after 21 d of remodeling and hypertrophy. These results demonstrate that CFR is reduced in mice as in humans with cardiac disease but before the onset of decompensated heart failure.
Craig J Hartley; Anilkumar K Reddy; Sridhar Madala; Lloyd H Michael; Mark L Entman; George E Taffet
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-02-06
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  34     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-21     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  892-901     Citation Subset:  IM    
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MeSH Terms
Aortic Valve / ultrasonography
Blood Flow Velocity
Cardiomegaly / physiopathology,  ultrasonography*
Coronary Vessels / physiopathology,  ultrasonography*
Hyperemia / physiopathology,  ultrasonography
Mice, Inbred C57BL
Mitral Valve / ultrasonography
Models, Animal
Regional Blood Flow
Ultrasonography, Doppler / methods*
Ventricular Dysfunction, Left / physiopathology,  ultrasonography
Grant Support
K25 HL073041/HL/NHLBI NIH HHS; K25 HL073041-03/HL/NHLBI NIH HHS; K25 HL073041-04/HL/NHLBI NIH HHS; K25-HL73041/HL/NHLBI NIH HHS; P01 HL042550/HL/NHLBI NIH HHS; P01 HL042550-119005/HL/NHLBI NIH HHS; P01 HL042550-129005/HL/NHLBI NIH HHS; P01 HL042550-139005/HL/NHLBI NIH HHS; P01 HL042550-13S19005/HL/NHLBI NIH HHS; P01 HL042550-149005/HL/NHLBI NIH HHS; P01 HL042550-15/HL/NHLBI NIH HHS; P01 HL042550-150001/HL/NHLBI NIH HHS; P01 HL042550-159005/HL/NHLBI NIH HHS; P01-HL42550/HL/NHLBI NIH HHS; R01 AG017899-01A2/AG/NIA NIH HHS; R01 AG017899-02/AG/NIA NIH HHS; R01 AG017899-03/AG/NIA NIH HHS; R01 AG017899-04/AG/NIA NIH HHS; R01 AG017899-05/AG/NIA NIH HHS; R01 HL022512/HL/NHLBI NIH HHS; R01 HL022512-24/HL/NHLBI NIH HHS; R01 HL022512-25/HL/NHLBI NIH HHS; R01 HL022512-26/HL/NHLBI NIH HHS; R01 HL022512-27/HL/NHLBI NIH HHS; R01 HL022512-28/HL/NHLBI NIH HHS; R01 HL022512-29/HL/NHLBI NIH HHS; R01 HL022512-30/HL/NHLBI NIH HHS; R01-AG17899/AG/NIA NIH HHS; R01-HL22512/HL/NHLBI NIH HHS; R03 AG015568-01/AG/NIA NIH HHS; R37 HL022512/HL/NHLBI NIH HHS; R37 HL022512-22/HL/NHLBI NIH HHS; R37 HL022512-23/HL/NHLBI NIH HHS; R41 HL076928-01A1/HL/NHLBI NIH HHS; R41-HL76928/HL/NHLBI NIH HHS; S10 RR014799/RR/NCRR NIH HHS; S10 RR014799-01/RR/NCRR NIH HHS
Reg. No./Substance:
0/Anesthetics; CYS9AKD70P/Isoflurane

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