| Dopamine transport sites selectively labeled by a novel photoaffinity probe: 125I-DEEP. | |
MedLine Citation:
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PMID: 2769361 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The dopamine transporter was labeled using a photosensitive compound related to GBR-12909, 125I-1-[2-(diphenylmethoxy)ethyl]-4-[2- (4-azido-3-iodophenyl)ethyl]piperazine (125I-DEEP). 125I-DEEP bound reversibly and with high affinity to the dopamine transport protein in the absence of light and could be covalently attached to the protein following exposure to UV light. In rat striatal homogenates, 125I-DEEP was found to incorporate covalently into a protein with apparent molecular weight of 58,000 Da. The properties of this binding protein were characteristic of the dopamine transporter since covalent attachment could be inhibited by dopamine-uptake blockers with the proper pharmacological rank order of potencies. Covalent binding was also inhibited in a stereospecific manner by (+) and (-) cocaine, as well as other cocaine analogs. The protein was not found in the cerebellum. The dopamine transporter appears to exist in a glycosylated form since photoaffinity-labeled transport sites could adsorb to wheat germ-agglutinin and could be specifically eluted from the column by beta-N-acetylglucosamine. |
Authors:
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D E Grigoriadis; A A Wilson; R Lew; J S Sharkey; M J Kuhar |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 9 ISSN: 0270-6474 ISO Abbreviation: J. Neurosci. Publication Date: 1989 Aug |
Date Detail:
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Created Date: 1989-09-22 Completed Date: 1989-09-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2664-70 Citation Subset: IM |
Affiliation:
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Neuroscience Branch, National Institute on Drug Abuse, Baltimore, Maryland 21224. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels Animals Azides / diagnostic use* Biological Transport Chemical Phenomena Chemistry Corpus Striatum / metabolism* Dopamine / metabolism* Iodine Radioisotopes / diagnostic use Male Membranes / metabolism Piperazines / diagnostic use* Proteins / metabolism Rats Rats, Inbred Strains Sepharose / diagnostic use Ultraviolet Rays / diagnostic use Wheat Germ Agglutinins / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Azides; 0/Iodine Radioisotopes; 0/Piperazines; 0/Proteins; 0/Wheat Germ Agglutinins; 123632-48-4/1-(2-(diphenylmethoxy)ethyl)-4-(2-(4-azido-3-iodophenyl)ethyl)piperazine; 9012-36-6/Sepharose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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