Document Detail


Dopamine transport sites selectively labeled by a novel photoaffinity probe: 125I-DEEP.
MedLine Citation:
PMID:  2769361     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dopamine transporter was labeled using a photosensitive compound related to GBR-12909, 125I-1-[2-(diphenylmethoxy)ethyl]-4-[2- (4-azido-3-iodophenyl)ethyl]piperazine (125I-DEEP). 125I-DEEP bound reversibly and with high affinity to the dopamine transport protein in the absence of light and could be covalently attached to the protein following exposure to UV light. In rat striatal homogenates, 125I-DEEP was found to incorporate covalently into a protein with apparent molecular weight of 58,000 Da. The properties of this binding protein were characteristic of the dopamine transporter since covalent attachment could be inhibited by dopamine-uptake blockers with the proper pharmacological rank order of potencies. Covalent binding was also inhibited in a stereospecific manner by (+) and (-) cocaine, as well as other cocaine analogs. The protein was not found in the cerebellum. The dopamine transporter appears to exist in a glycosylated form since photoaffinity-labeled transport sites could adsorb to wheat germ-agglutinin and could be specifically eluted from the column by beta-N-acetylglucosamine.
Authors:
D E Grigoriadis; A A Wilson; R Lew; J S Sharkey; M J Kuhar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  9     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-22     Completed Date:  1989-09-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2664-70     Citation Subset:  IM    
Affiliation:
Neuroscience Branch, National Institute on Drug Abuse, Baltimore, Maryland 21224.
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MeSH Terms
Descriptor/Qualifier:
Affinity Labels
Animals
Azides / diagnostic use*
Biological Transport
Chemical Phenomena
Chemistry
Corpus Striatum / metabolism*
Dopamine / metabolism*
Iodine Radioisotopes / diagnostic use
Male
Membranes / metabolism
Piperazines / diagnostic use*
Proteins / metabolism
Rats
Rats, Inbred Strains
Sepharose / diagnostic use
Ultraviolet Rays / diagnostic use
Wheat Germ Agglutinins / diagnostic use
Chemical
Reg. No./Substance:
0/Affinity Labels; 0/Azides; 0/Iodine Radioisotopes; 0/Piperazines; 0/Proteins; 0/Wheat Germ Agglutinins; 123632-48-4/1-(2-(diphenylmethoxy)ethyl)-4-(2-(4-azido-3-iodophenyl)ethyl)piperazine; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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