Document Detail


Dopamine-melanin is actively phagocytized by PC12 cells and cerebellar granular cells: possible implications for the etiology of Parkinson's disease.
MedLine Citation:
PMID:  10025709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuromelanin in the substantia nigra may be associated with the pathogenesis of nigral cell death in Parkinson's disease. We used synthetic dopamine-melanin (DA-M) as a model compound for neuromelanin and examined its toxic effects on mice cerebellar granule cells and a rat pheochromocytoma cell line (PC12). The DA-M and dopamine-treated cells showed an accumulation of black deposits when examined by light microscopy. Electron microscopy revealed different stages of DA-M phagocytosis, starting with DA-M binding, engulfment of the particles and the formation of phagosomes located in the cytoplasm. Using absorption assays, we found that NaN3 and low temperature inhibit the internalization of DA-M, pointing to an energy-dependent phagocytosis mechanism. These results suggest that neuromelanin can be phagocytised by neuronal cells which may thus be subjected to its toxic effects. These findings may contribute to our understanding of the formation and disposition of neuromelanin and its possible role in the etiology of Parkinson's disease.
Authors:
D Offen; S Gorodin; E Melamed; J Hanania; Z Malik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  260     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-06-18     Completed Date:  1999-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  101-4     Citation Subset:  IM    
Affiliation:
Department of Neurology and Felsenstein Medical Research Center, Rabin Medical Center, Petah-Tiqva, Israel. offen-d@netvision.net.il
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebellar Cortex / cytology,  physiology
Granulocytes / drug effects,  physiology*
Melanins / metabolism*
Mice
PC12 Cells / drug effects,  physiology*
Parkinson Disease / etiology*
Phagocytosis / physiology*
Rats
Chemical
Reg. No./Substance:
0/Melanins; 0/dopamine melanin; 0/neuromelanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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