Document Detail

Dopamine and its metabolites in human tears.
MedLine Citation:
PMID:  8353435     Owner:  NLM     Status:  MEDLINE    
Dopamine is known to stimulate ion transport in the corneal epithelium. Since epithelium is in contact with tears, we searched for dopamine and its metabolites in tears. Twenty normal subjects participated in this study. Twenty microliters of tears were collected in capillary tubes after trigeminal stimulation and immediately analyzed using high-performance liquid chromatography with electrochemical detection. Dopamine and two of its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), were identified and measured. The mean concentration of dopamine was 8.9 +/- 5.1 ng/ml; DOPAC, 2.2 +/- 1.6 ng/ml; and HVA, 0.55 +/- 0.4 ng/ml. These results permit us to suggest that dopamine is deaminated by monoamine oxidase, then methylated by catechol-O-methyltransferase and that catabolism occurs in the lacrimal gland and perhaps in corneal epithelium. Finally, part of the dopamine involved in ion transport may come from tears.
X D Martin; M C Brennan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of ophthalmology     Volume:  3     ISSN:  1120-6721     ISO Abbreviation:  Eur J Ophthalmol     Publication Date:    1993 Apr-Jun
Date Detail:
Created Date:  1993-09-23     Completed Date:  1993-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9110772     Medline TA:  Eur J Ophthalmol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  83-8     Citation Subset:  IM    
Department of Ophthalmology, University of Zurich, Switzerland.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis*
Chromatography, High Pressure Liquid
Dopamine / analysis*
Homovanillic Acid / analysis*
Middle Aged
Tears / chemistry*
Reg. No./Substance:
102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid

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