Document Detail


Dopamine-deficient mice are severely hypoactive, adipsic, and aphagic.
MedLine Citation:
PMID:  8548806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mice unable to synthesize dopamine (DA) specifically in dopaminergic neurons were created by inactivating the tyrosine hydroxylase (TH) gene then by restoring TH function in noradrenergic cells. These DA-deficient (DA-/-) mice were born at expected frequency but became hypoactive and stopped feeding a few weeks after birth. Midbrain dopaminergic neurons, their projections, and most characteristics of their target neurons in the striatum appeared normal. Within a few minutes of being injected with L-dihdroxyphenylalanine (L-DOPA), the product of TH, the DA-/- mice became more active and consumed more food than control mice. With continued administration of L-DOPA, nearly normal growth was achieved. These studies indicate that DA is essential for movement and feeding, but is not required for the development of neural circuits that control these behaviors.
Authors:
Q Y Zhou; R D Palmiter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  83     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-02-21     Completed Date:  1996-02-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1197-209     Citation Subset:  IM    
Affiliation:
Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle 98195-7370, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic Fibers / metabolism
Animals
Animals, Newborn
Behavior, Animal / physiology*
Dopamine / analysis,  deficiency*
Dopamine beta-Hydroxylase / metabolism
Drinking Behavior / physiology*
Dynorphins / biosynthesis
Embryo, Mammalian / physiology
Feeding Behavior / physiology*
Immunohistochemistry
In Situ Hybridization
Levodopa / pharmacology
Mesencephalon / enzymology
Mice
Mice, Transgenic
Mutation / physiology
Neostriatum / cytology,  metabolism
Neurons / chemistry,  cytology,  physiology
Norepinephrine / biosynthesis,  deficiency
Substance P / biosynthesis
Transgenes / physiology
Tyrosine 3-Monooxygenase / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HD-09172/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Levodopa; 33507-63-0/Substance P; 51-41-2/Norepinephrine; 74913-18-1/Dynorphins; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 1.14.17.1/Dopamine beta-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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