Document Detail

Donor-specific immune response after aortic valve allografting in the rat.
MedLine Citation:
PMID:  8179379     Owner:  NLM     Status:  MEDLINE    
The allospecific immune response in rats to a major histocompatibility complex-disparate aortic valve allograft was investigated using three in vitro assays. In each assay, DA strain (RT-1a) rats served as allograft recipient and syngeneic donor, Lewis strain (RT-1l) rats were allogeneic donors, and Buffalo (RT-1b) rats provided third-party control cells. Mixed lymphocyte cultures using spleen cells demonstrated donor-specific stimulation indices of 3.04 +/- 0.44, 4.14 +/- 0.62, and 6.32 +/- 0.60 at 7, 14, and 28 days, respectively, after aortic valve allografting; 8.19 +/- 2.91, 8.51 +/- 1.25, and 10.80 +/- 0.53 after skin allografting; and 1.84 +/- 0.56, 1.82 +/- 0.38, and 1.82 +/- 0.53 after aortic valve isografting. Limiting dilution analysis of splenocytes showed a donor-specific cytotoxic T lymphocyte precursor frequency at 7, 14, and 28 days of 1:6,853, 1:4,714, and 1:1,964 after aortic valve allografting; 1:4,181, 1:1,611, and 1:1,018 after skin allografting; and 1:14,517, 1:11,882, and 1:10,995 after aortic valve isografting. Flow cytometry detected an increase in the level of donor-specific anti-T cell antibodies in both valve and skin allograft recipients but not in isografted animals. Aortic valve allografting from Lewis into DA rats elicits allospecific cellular and humoral immune responses similar in magnitude to skin allografting but somewhat slower in onset. Investigation of the immune response to aortic allografts in humans is warranted, as donor-specific T cells, antibodies, or both may damage the allograft.
X M Zhao; M Green; I H Frazer; P Hogan; M F O'Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  57     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-08     Completed Date:  1994-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1158-63     Citation Subset:  AIM; IM    
Lions Human Immunology Laboratories, Princess Alexandra Hospital, University of Queensland, Brisbane, Australia.
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MeSH Terms
Aortic Valve / immunology,  transplantation*
Flow Cytometry
Isoantibodies / analysis
Lymphocyte Culture Test, Mixed
Rats, Inbred BUF
Rats, Inbred Lew
Rats, Inbred Strains
Skin Transplantation / immunology
T-Lymphocyte Subsets
T-Lymphocytes, Cytotoxic
Transplantation Immunology*
Transplantation, Homologous / immunology
Transplantation, Isogeneic / immunology
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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