Document Detail

Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds.
MedLine Citation:
PMID:  23209565     Owner:  NLM     Status:  MEDLINE    
The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were isolated and cultured in the presence of osteogenic, neurogenic, or vasculogenic differentiation medium. Proliferation ability was evaluated by determining doubling time, and commitment ability was evaluated by gene expression and morphological analyses for tissue-specific markers. The results confirm a well-defined proliferative ability for each donor age group at an early in vitro passage (p2). DPSCs from younger donors (up to 35 years) maintain this ability in long-term cultures (p8). Stem cells of all age donor groups maintain their commitment ability during in vitro culture. In vivo tests on the critical size defect repair process confirmed that DPSCs of all donor ages are a potent tool for bone tissue regeneration when mixed with 3D nanostructured scaffolds.
Eriberto Bressan; Letizia Ferroni; Chiara Gardin; Paolo Pinton; Edoardo Stellini; Daniele Botticelli; Stefano Sivolella; Barbara Zavan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-28
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-04     Completed Date:  2013-06-11     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e49146     Citation Subset:  IM    
Department of Neurosciences, University of Padua, Padua, Italy.
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MeSH Terms
Adult Stem Cells / cytology*,  metabolism*
Age Factors
Bone Regeneration
Cell Culture Techniques
Cell Differentiation
Cell Lineage / genetics
Cell Proliferation
Dental Pulp / metabolism*
Endothelial Cells / cytology,  metabolism
Gene Expression Regulation
Middle Aged
Neurons / cytology,  metabolism
Osteogenesis / genetics
Tissue Donors
Tissue Scaffolds
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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