| Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 1097402 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Different dominant thermosensitive mutations affecting the same gene were selected in Saccharomyces cerevisiae. Ribonucleic acid (RNA) synthesis decreased rapidly and markedly at 37 C in all the mutants whether they were in a homozygous or a heterozygous state. Protein biosynthesis was at first unaffected and then decreased slowly, stopping after 5 h. Measurements of RNA biosynthesis in isolated nuclei as well as in vitro activities of RNA polymerases A and B at 22 and 37 C failed to reveal any difference between mutants and the wild type. Analysis of the nature of the residual RNAs synthesized at the high temperature in the mutants showed a small relative increase in the messenger RNA fraction, but it was not sufficient to indicate a specific inactivation of RNA polymerase A activity. The results suggest an impairment in a common regulatory element for all RNA polymerases acting at the level of the initiation of transcription. Similar mutants with a semidominant phenotype were obtained in which the lesions were in two other unlinked loci. |
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Authors:
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F Lacroute; J Huet; F Exinger |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 122 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1975 Jun |
Date Detail:
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Created Date: 1975-11-06 Completed Date: 1975-11-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 847-54 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antifungal Agents
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pharmacology Cell Nucleus / metabolism Cell-Free System Cycloheximide / pharmacology DNA-Directed RNA Polymerases / metabolism Fungal Proteins / biosynthesis Genes, Dominant* Isoenzymes / metabolism Mutation* Nitrates / pharmacology Phenazines / pharmacology Phenotype Pyrroles / pharmacology RNA / biosynthesis* Saccharomyces cerevisiae / enzymology, metabolism* Temperature Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Fungal Proteins; 0/Isoenzymes; 0/Nitrates; 0/Phenazines; 0/Pyrroles; 63231-63-0/RNA; 66-81-9/Cycloheximide; EC 2.7.7.6/DNA-Directed RNA Polymerases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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