Document Detail

Dominant negative pleiotrophin induces tetraploidy and aneuploidy in U87MG human glioblastoma cells.
MedLine Citation:
PMID:  17067552     Owner:  NLM     Status:  MEDLINE    
Pleiotrophin (PTN, Ptn) is an 18kDa secretory cytokine that is expressed in many human cancers, including glioblastoma. In previous experiments, interruption of the constitutive PTN signaling in human U87MG glioblastoma cells that inappropriately express endogenous Ptn reversed their rapid growth in vitro and their malignant phenotype in vivo. To seek a mechanism for the effect of the dominant-negative PTN, flow cytometry was used to compare the profiles of U87MG cells and four clones of U87MG cells that express the dominant-negative PTN (U87MG/PTN1-40 cells); here, we report that the dominant-negative PTN in U87MG cells induces tetraploidy and aneuploidy and arrests the tetraploid and aneuploid cells in the G1 phase of the cell cycle. The data suggest that PTN signaling may have a critical role in chromosomal segregation and cell cycle progression; the data suggest induction of tetraploidy and aneuploidy in U87MG glioblastoma cells may be an important mechanism that contributes to the loss of the malignant phenotype of U87MG cells.
Yunchao Chang; James R Berenson; Zhaoyi Wang; Thomas F Deuel
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-10-05
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  351     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-30     Completed Date:  2007-01-19     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  336-9     Citation Subset:  IM    
Department of Molecular and Experimental Medicine, the Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
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MeSH Terms
Carrier Proteins / physiology*
Cell Cycle / physiology*
Cell Line, Tumor
Cloning, Molecular
Cytokines / physiology*
Flow Cytometry
G1 Phase / physiology
Grant Support
R01 CA084400-06/CA/NCI NIH HHS; R01 CA84328/CA/NCI NIH HHS; R01 CA84400/CA/NCI NIH HHS
Reg. No./Substance:
0/Carrier Proteins; 0/Cytokines; 134034-50-7/pleiotrophin

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