| Dominant negative pleiotrophin induces tetraploidy and aneuploidy in U87MG human glioblastoma cells. | |
| | |
MedLine Citation:
|
PMID: 17067552 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pleiotrophin (PTN, Ptn) is an 18kDa secretory cytokine that is expressed in many human cancers, including glioblastoma. In previous experiments, interruption of the constitutive PTN signaling in human U87MG glioblastoma cells that inappropriately express endogenous Ptn reversed their rapid growth in vitro and their malignant phenotype in vivo. To seek a mechanism for the effect of the dominant-negative PTN, flow cytometry was used to compare the profiles of U87MG cells and four clones of U87MG cells that express the dominant-negative PTN (U87MG/PTN1-40 cells); here, we report that the dominant-negative PTN in U87MG cells induces tetraploidy and aneuploidy and arrests the tetraploid and aneuploid cells in the G1 phase of the cell cycle. The data suggest that PTN signaling may have a critical role in chromosomal segregation and cell cycle progression; the data suggest induction of tetraploidy and aneuploidy in U87MG glioblastoma cells may be an important mechanism that contributes to the loss of the malignant phenotype of U87MG cells. |
| | |
Authors:
|
Yunchao Chang; James R Berenson; Zhaoyi Wang; Thomas F Deuel |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-10-05 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 351 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-11-30 Completed Date: 2007-01-19 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 336-9 Citation Subset: IM |
Affiliation:
|
Department of Molecular and Experimental Medicine, the Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aneuploidy* Carrier Proteins / physiology* Cell Cycle / physiology* Cell Line, Tumor Cloning, Molecular Cytokines / physiology* Flow Cytometry G1 Phase / physiology Glioblastoma Humans Mutation Polyploidy* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 CA084400-06/CA/NCI NIH HHS; R01 CA84328/CA/NCI NIH HHS; R01 CA84400/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Carrier Proteins; 0/Cytokines; 134034-50-7/pleiotrophin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Direct visualization of actin nematic network formation and dynamics.
Next Document: Resistin increases lipid accumulation and CD36 expression in human macrophages.