Document Detail

Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes.
MedLine Citation:
PMID:  19148472     Owner:  NLM     Status:  MEDLINE    
We previously reported that the inactivation of the Ets 1 transcription factor by a specific decoy strategy reduces rat C6 glioma cell proliferation and mmp-9 expression. In the present study, we analysed the effects of the dominant-negative form of Ets 1 (Ets-DB) on rat C6 glioma cell proliferation, migration, invasion, in vivo tumor growth on the chicken chorioallantoic membrane (CAM) and mmp-9 expression. In addition, we examined differences in gene expression between Ets-DB expressing and control cells using suppression subtractive hybridization (SSH). We found that retrovirus mediated expression of Ets-DB inhibited cellular proliferation, migration, invasion, mmp-9 expression, cellular growth in soft agar, and in vivo growth in the chicken chorioallantoic membrane assay. SSH analysis revealed expression of different genes in Ets-DB expressing cells involved in basic cellular processes. Each of these genes contained binding sites for different Ets-factors within their promoters. Finally, we found that, in addition to Ets 1, Elk-1, Elf-1, Fli-1 and Etv-1 are further Ets family members expressed in rat C6 glioma cells. Our results indicate that Ets transcription factors play important roles for basic properties of rat C6 glioma cells. Targeting of these factors might therefore become a useful experimental tool for therapeutic strategies against malignant gliomas.
Ayguen Sahin; Chantal Vercamer; Annette Kaminski; Tanja Fuchs; Alexandra Florin; Jens Claus Hahne; Virginie Mattot; Albin Pourtier-Manzanedo; Torsten Pietsch; Véronique Fafeur; Nicolas Wernert
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  34     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  377-89     Citation Subset:  IM    
Institute of Pathology, Department of Neuropathology, University of Bonn, 53011 Bonn, Germany.
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MeSH Terms
Binding Sites
Cell Division
Cell Line, Tumor
Chick Embryo
DNA Primers
Gene Expression Regulation, Neoplastic
Glioma / enzymology,  genetics*,  pathology*
Matrix Metalloproteinase 9 / genetics
Neoplasm Invasiveness
Neoplasm Proteins / genetics
Proto-Oncogene Protein c-ets-1 / antagonists & inhibitors*,  genetics*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/DNA Primers; 0/Neoplasm Proteins; 0/Proto-Oncogene Protein c-ets-1; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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