| Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes. | |
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MedLine Citation:
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PMID: 19148472 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that the inactivation of the Ets 1 transcription factor by a specific decoy strategy reduces rat C6 glioma cell proliferation and mmp-9 expression. In the present study, we analysed the effects of the dominant-negative form of Ets 1 (Ets-DB) on rat C6 glioma cell proliferation, migration, invasion, in vivo tumor growth on the chicken chorioallantoic membrane (CAM) and mmp-9 expression. In addition, we examined differences in gene expression between Ets-DB expressing and control cells using suppression subtractive hybridization (SSH). We found that retrovirus mediated expression of Ets-DB inhibited cellular proliferation, migration, invasion, mmp-9 expression, cellular growth in soft agar, and in vivo growth in the chicken chorioallantoic membrane assay. SSH analysis revealed expression of different genes in Ets-DB expressing cells involved in basic cellular processes. Each of these genes contained binding sites for different Ets-factors within their promoters. Finally, we found that, in addition to Ets 1, Elk-1, Elf-1, Fli-1 and Etv-1 are further Ets family members expressed in rat C6 glioma cells. Our results indicate that Ets transcription factors play important roles for basic properties of rat C6 glioma cells. Targeting of these factors might therefore become a useful experimental tool for therapeutic strategies against malignant gliomas. |
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Authors:
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Ayguen Sahin; Chantal Vercamer; Annette Kaminski; Tanja Fuchs; Alexandra Florin; Jens Claus Hahne; Virginie Mattot; Albin Pourtier-Manzanedo; Torsten Pietsch; Véronique Fafeur; Nicolas Wernert |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 34 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-16 Completed Date: 2009-04-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 377-89 Citation Subset: IM |
Affiliation:
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Institute of Pathology, Department of Neuropathology, University of Bonn, 53011 Bonn, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allantois Animals Binding Sites Cell Division Cell Line, Tumor Chick Embryo Chorion DNA Primers Gene Expression Regulation, Neoplastic Glioma / enzymology, genetics*, pathology* Matrix Metalloproteinase 9 / genetics Neoplasm Invasiveness Neoplasm Proteins / genetics Proto-Oncogene Protein c-ets-1 / antagonists & inhibitors*, genetics*, metabolism Rats Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Neoplasm Proteins; 0/Proto-Oncogene Protein c-ets-1; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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