Document Detail


Dominant-negative abrogation of connexin-mediated cell growth control by mutant connexin genes.
MedLine Citation:
PMID:  9393973     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Connexin genes exert negative growth control when transfected into various types of tumor cell lines. We previously demonstrated that connexin 26 (Cx26) suppresses in vitro and in vivo growth of HeLa cells. In this study, we have examined whether certain Cx26 mutants can abrogate cell growth control and the gap junctional intercellular communication (GJIC) capacity of such Cx26-transfected HeLa cells. For this purpose, we transfected three mutated Cx26 genes (C60F, P87L and R143W) into HeLa cells already containing the wild-type Cx26 gene, which are GJIC-competent and non-tumorigenic. Transfection of P87L and R143W mutants enhanced the tumorigenicity of the HeLa Cx26 cells in nude mice without any change in GJIC capacity. On the other hand, transfection of the C60F mutant reduced the GJIC capacity of HeLa Cx26 cells without affecting their growth in vivo. Immunostaining studies demonstrated that the Cx26 proteins were localized mainly at cell-cell contact areas in the HeLa Cx26 cells both before and after transfection of mutated Cx26 genes. These results suggest that certain mutant Cx26 proteins exert a dominant-negative effect on Cx26-regulated growth of HeLa cells and that such effects may be independent of the effect on GJIC ability. It is proposed that wild-type and mutant Cx26 proteins produce heteromeric connexons and that such heteromeric connexons may exert different effects on growth control from those of homomeric connexons.
Authors:
A Duflot-Dancer; M Mesnil; H Yamasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  15     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2151-8     Citation Subset:  IM    
Affiliation:
Unit of Multistage Carcinogenesis, International Agency for Research on Cancer, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology
Cell Division / physiology
Connexins / genetics,  physiology*
Gap Junctions / physiology
Hela Cells
Humans
Mice
Mice, Nude
Mutation*
Neoplasms / genetics*,  pathology*
Polymerase Chain Reaction
RNA / analysis
Rats
Transcription, Genetic
Transfection
Xenopus
Grant Support
ID/Acronym/Agency:
R01-CA-40534/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Connexins; 127120-53-0/connexin 26; 63231-63-0/RNA

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