Document Detail

Dominant negative ATF1 blocks cyclic AMP-induced neurite outgrowth in PC12D cells.
MedLine Citation:
PMID:  9489722     Owner:  NLM     Status:  MEDLINE    
Extension of the neuronal process is a crucial step for establishment of the neuronal network. As CREB preferentially forms heterodimers with ATF1 in PC12D cells, we examined the roles of the CREB/ATF1 heterodimer on cyclic AMP (cAMP)-induced neurite extension, using originally constructed ATF1RL, which has a point mutation at the DNA binding domain of ATF1. Transient expression of ATF1RL suppressed the protein kinase A/CREB-induced expression of the CRE reporter gene as expected. Treatment with forskolin elicited a relatively poor mRNA induction for immediate early genes in PC12D-ATF1 RL cells, a PC12D cell line stably expressing ATF1RL, in comparison with the parental PC12D cells. Furthermore, the PC12D-ATF1RL cells were proved to be defective at cAMP-induced neurite outgrowth. In contrast, both the gene expression and the differentiation after nerve growth factor treatment noted in PC12D-ATF1RL cells were at the same levels as those in the parental cells. These data provide us the first evidence that links CREB/ATF1 to the cAMP-induced differentiation of PC12 cells.
A Shimomura; Y Okamoto; Y Hirata; M Kobayashi; K Kawakami; K Kiuchi; T Wakabayashi; M Hagiwara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  70     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-03-12     Completed Date:  1998-03-12     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1029-34     Citation Subset:  IM    
Department of Anatomy, Nagoya University School of Medicine, Japan.
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MeSH Terms
Activating Transcription Factor 1
Cell Differentiation / drug effects,  physiology
Cyclic AMP / pharmacology*
Cyclic AMP Response Element-Binding Protein / genetics
DNA-Binding Proteins*
Gene Expression / drug effects,  physiology
Genes, Immediate-Early / genetics
Mutagenesis / physiology
Neurites / drug effects,  physiology*
Neurons / cytology*,  ultrastructure
PC12 Cells
Recombinant Proteins / pharmacology
Transcription Factors / genetics*
Transcription, Genetic / drug effects,  physiology
Reg. No./Substance:
0/Activating Transcription Factor 1; 0/Cyclic AMP Response Element-Binding Protein; 0/DNA-Binding Proteins; 0/Recombinant Proteins; 0/Transcription Factors; 60-92-4/Cyclic AMP

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