Document Detail


Dominant uptake of fatty acid over glucose by prostate cells: a potential new diagnostic and therapeutic approach.
MedLine Citation:
PMID:  20332441     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Prostate cancer is characterized by a low rate of glycolysis and glucose uptake. We hypothesize that fatty acid is dominant over glucose in uptake by prostate cells. MATERIALS AND METHODS: One benign (RWPE1) and two malignant (LNCaP and PC3) prostate cell lines were assayed for their in vitro uptake of radiolabeled glucose analogs (3)H-fluoro-2-deoxyglucose and (18)F-fluoro-2-deoxyglucose, and the long-chain fatty acid (3)H-palmitic acid. Fractional uptake was standardized to viable cell numbers. RESULTS: Uptake of palmitate in all 3 prostate cell lines was significantly higher than that of glucose at all incubation times (p<0.01). But in malignant cell lines, neither glucose nor palmitate uptake was quantitatively higher than that in the benign cell line. The uptake of fatty acid by prostate cells is a dynamic, active process mediated by the membrane receptors. CONCLUSION: Prostate cells are characterized by a dominant uptake of fatty acid over glucose, suggesting that future development of new diagnostic and therapeutic approaches in prostatic cancer should focus on fatty acid substrate. Fatty acid imaging may be useful in detection of recurrence and metastasis, but not in differentiating malignant from benign prostate tissue.
Authors:
Yiyan Liu; Lionel S Zuckier; Nasrin V Ghesani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  369-74     Citation Subset:  IM    
Affiliation:
Section of Nuclear Medicine, Department of Radiology, 150 Bergen Street, H-141, New Jersey Medical School, UMDNJ, Newark, New Jersey 07103, USA. liuyl@umdnj.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Survival
Cells, Cultured
Fluorine Radioisotopes / metabolism
Fluorodeoxyglucose F18 / metabolism
Glucose / metabolism*
Humans
Male
Palmitic Acids / metabolism*
Prostate / metabolism*,  pathology,  radionuclide imaging
Prostatic Neoplasms / metabolism*,  pathology,  radionuclide imaging
Sweetening Agents / metabolism*
Chemical
Reg. No./Substance:
0/Fluorine Radioisotopes; 0/Palmitic Acids; 0/Sweetening Agents; 50-99-7/Glucose; 63503-12-8/Fluorodeoxyglucose F18

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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