| Domains of apoE4 required for the biogenesis of apoE-containing HDL. | |
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MedLine Citation:
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PMID: 21604997 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract Introduction. We have studied the functions of truncated apoE4 forms in vitro and in vivo in order to identify the domains of apoE4 required for the biogenesis of apoE-containing high-density lipoprotein (HDL). Results. We have found that apoE4-185, -202, -229, or -259 could promote ATP-binding cassette transporter A1 (ABCA1)-dependent cholesterol efflux in vitro, although less efficiently than Full-length apoE4, and had diminished capacity to activate lecithin cholesterol acyltransferase (LCAT). Formation of HDL in vivo was assessed by various methods following gene transfer in apolipoprotein A-I(-/-) × apoE(-/-) mice. Fast protein liquid chromatography of plasma showed that the truncated apoE forms, except apoE4-185, generated an apoE-containing HDL peak. Two-dimensional gel electrophoresis of plasma and electron microscopy showed that truncated apoE forms generated distinct HDL subpopulations and formed discoidal HDL particles which could be converted to spherical by co-administration of truncated apoE4-202 and LCAT. Conclusion. Overall, the in-vivo and in-vitro data are consistent and indicate that apoE4-185 is the shortest truncated form that supports formation of discoidal apoE4-containing HDL particles. |
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Authors:
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Alexander M Vezeridis; Angeliki Chroni; Vassilis I Zannis |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of medicine Volume: 43 ISSN: 1365-2060 ISO Abbreviation: Ann. Med. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8906388 Medline TA: Ann Med Country: England |
Other Details:
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Languages: eng Pagination: 302-11 Citation Subset: IM |
Affiliation:
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Molecular Genetics, Whitaker Cardiovascular Institute, Boston University School of Medicine, 700 Albany Street W509, Boston MA 02118, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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